Genomics Kits

The GenieClone cloning technology is simple, fast, and highly efficient. It enables directional insertion of any amplified DNA product into any linearized vector at any site.

Firstly, the vector is first linearized at the cloning site. A small overlapping sequence at each end of the cloning site is added to the insert through PCR. The insert and the linearized vector both with overlapping 5’- and 3’ (15 bp - 20 bp) sequences are mixed in an appropriate ratio and incubated with GenieClone Recombinase at 50℃ for 5 - 15 min before transformation of competent cells for successful DNA cloning and assembly.

GenieClone UItra One Step Cloning kit allows for the highly efficient cloning and assembly of 1-5 DNA fragments in as little as 15 minutes into any vector at any site. This system utilizes ligation-independent technology and a novel GenieClone recombinase to significantly reduce self-ligating colonies. The enhanced GenieClone Recombinase and highly optimized buffer included in the 2x GenieClone Mix significantly improve the recombination efficiency and the tolerance to impurities. The pCE-ONE vector is compatible with most PCR products, enabling the specific PCR fragments to be used directly for recombination without any pre-treatment.

Figure: Mechanism of GenieClone Simple Homologous Recombination

• Fast Cloning
• High-throughput Cloning
• Seamless Assembly
• Site-specific Mutagenesis

Genie Fusion Ultra High-Fidelity DNA Polymerase is a next generation enzyme for robust PCR with ultra high-fidelity. With extremely low error rates x53-fold lower than Taq, Genie Fusion Ultra High Fidelity DNA Polymerase sets a new level of performance for standard and long fragment PCR amplification.

Employing a unique extension factor as well as specificity-promoting and plateau-inhibiting factors Genie Fusion greatly improves long range amplification ability, specificity, and PCR yield. Genie Fusion is capable of amplifying long fragments such as 40 kb λ DNA, 40 kb plasmid DNA, 20 kb genomic DNA and 10 kb cDNA. In addition, Genie Fusion has a excellent resistance to PCR inhibitors and can be used for direct PCR amplifications of bacteria, fungi, plant tissues, animal tissues, and even whole blood samples. Genie Fusion contains two monoclonal antibodies inhibiting the 5´→3´ polymerase activity and 3´→5´ exonuclease activity at room temperature which enables Genie Fusion to perform super specific Hot-Start PCRs. The amplification generates blunt-ended products, which are compatible with GenieClone DNA Assembly Cloning Kit (Assay Genie, #MORV0102)

Figure: Using mouse tail lysate as a template, a target fragment of 2.5 kb was amplified with Genie Fusion Ultra High-Fidelity DNA Polymerase, a high-fidelity DNA polymerase from company A, and a high-fidelity DNA polymerase from company B. The Tm of all primers are approximately 60℃ (calculated in Primer Premier 5). ( M: 5kb DNA Marker, 1: Tomato leaf, 2: Rice leaf, 3: Polished rice, 4: Purified genomic DNA from rice leaf.)

• Ultra high-fidelity DNA polymerase with error rates x53-fold lower than conventional Taq and x6-fold lower than Pfu.
• Excels at amplifying long fragments such as 40 kb λ DNA, 40 kb plasmid DNA, 20 kb genomic DNA and 10 kb cDNA.
• Superior performance with GC-rich templates.
• Extreme resistance to PCR inhibitors.
• Excellent choice for direct PCR from bacteria, fungi, plant tissues, animal tissues and whole blood samples.
• Formulated with dual monoclonal antibodies for super specific Hotstart PCR.
• Compatible with GenieClone DNA Assembly Cloning Kit (MORV0102).

GenieClone Site-Directed Mutagenesis Kit is a site-directed mutagenesis system based on GenieClone rapid cloning technology. This kit is designed for rapid site-directed mutagenesis of 3 to 5 separate mutations in the same reaction in less than 3 hours. Based on GenieClone rapid cloning technology, this kit uses homologous recombination to replace the conventional annealing ring-forming reactions meaning much less template and a more flexible primer design strategy.

With seamless splicing of the two PCR products by GenieClone technology, the kit can complete up to two separate mutations with a single amplification reaction. DpnI digested products of the specific amplicons can be directly added to the recombination reaction without purification. The highly optimized reaction buffer, fast protocol and superior site-directed mutagenesis efficiency make GenieClone Site-Directed Mutagenesis Kit v1 make an excellent choice for introducing 3 to 5 mutations at discontinuous sites simultaneously on the same plasmid.

Figure 1: Single base site-directed mutation using GenieClone Site-Directed Mutagenesis Kit v1

• Introduce 3 - 5 separate mutations in the same reaction.
• Site-directed mutagenesis can be performed on up to 5 discontinuous sites (more than 50 bp apart) simultaneously on the target plasmid.
• Single kit for cloning and mutagenesis.
• Includes Genie Fusion Ultra High-Fidelity DNA Polymerase providing high-fidelity PCR with extremely low mutation rates & excellent long fragment amplification for any plasmid amplification up to 20 kb as well as GC-rich templates.
• Amplification is carried out exponentially and the template usage is extremely low, which is beneficial to the complete degradation of the original methylation template.
• DpnI eliminates contamination of the original template and decreases background.
• Amplified products can be directly used in the recombination reaction after DpnI treatment.
• The reaction mixture can be used to directly transform chemically competent E.coli cells.

The MycoGenie Rapid Mycoplasma Detection Kit enables the rapid and simple detection of mycoplasma contamination in cell culture. This novel system detects mycoplasma in 1 hour from 1µl of cell culture supernatant by visual determination eliminating the requirement for PCR, qPCR, electrophoresis or ELISA. Compared with PCR, the MycoGenie Rapid Mycoplasma Detection Kit is more resistant cell culture inhibitors thus avoiding false positive and false negative results. The results are highly consistent with the most sensitive and accurate PCR kits for Mycoplasma detection.

The MycoGenie Rapid Mycoplasma Detection Kit can detect up to 28 mycoplasma species, including 8 of the most common species associated with cell culture contamination. It is suitable for mycoplasma detection in a wide range of suspension and adherent cells including CHO, Vero, hybridoma, Sf9, HEK293 and many more. Furthermore, the kit is compatible with a comprehensive suite of cell culture media and serum.

The MycoGenie Rapid Mycoplasma Detection Kit is an excellent choice for routine mycoplasma detection in biopharma, vaccine/monoclonal antibody production, cell therapy/embryo laboratories and other scientific research laboratories.

MycoGenie Rapid Mycoplasma Detection Kit is suitable for many kinds of suspension and adherent cells with a wide range of media and serum compatibility. These include but are not limited to:

MycoGenie MycoPlasma Elimination Kit is designed to eliminate Mycoplasma from contaminated cell culture media and serum.

Unlike conventional antibiotics used to treat mycoplasma contamination, this kit removes mycoplasma by disrupting membrane structure allowing elimination of even antibiotic-resistant mycoplasma. This is a much more effective method of eliminating mycoplasma versus other common methods that simply inhibit proliferation. It is also very effective at removing Gram-negative and positive bacteria. This kit successfully eliminates intracellular and extracellular mycoplasma after a 3-7 day treatment protocol. Furthermore, it is non-toxic to cells and has been shown to kill most mycoplasma species. The MycoGenie MycoPlasma Elimination Kit has been validated with most commonly used cell lines including mouse and human embryonic stem cells, iPS cells, HEK293, Hela, HepG2, HCT116, COS-7, Vero, Huh-7, MDCK, PANC-1, SW620 and U2OS.