Glycolysis Assay Kits
Glycolysis Assays
At Assay Genie we've developed a range of Glycolysis assays for researchers to analyse key steps in the glycolysis pathway.
Assay Genie's Glycolysis Assay Kit is based on measuring the production of L-Lactate from glycolysis in cells. L-Lactate that is secreted into the cell media when glycolysis occurs and is quantified using a coupled reaction involving the lactate dehydrogenase catalyzed oxidation of L-lactate that generates pyruvate and NADH which reduces a formazan dye.
The intensity of the reduced dye, measured at 565 nm, is directly proportional to the L-lactate concentration in the sample, which in turn is directly proportional to rate of glycolysis in the cells.
This assay can be used for the direct determination of L-Lactate produced by glycolysis in cell samples. Screening of glycolysis inhibitors.
Glycolysis Inhibition: PANC1 cells treated with varying concentrations of 2-deoxy-D-glucose in DMEM medium
Metabolites
| Assay | Code | Read-out | Sensitivity | Sample Type | Pack Size | ||
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Fluorometric |
50 pmol |
Cells, tissues |
100 Assays |
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BN00830 |
Colorimetric/Fluorometric |
100 pmol |
Serum, cells, tissues, urine |
100 Assays |
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Colorimetric |
1000 pmol |
Serum, cells, tissues, food |
100 Assays |
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Fluorometric |
10 pmol |
Serum, cells, tissues, food |
100 Assays |
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Colorimetric |
1000 pmol |
Serum, cells, tissues |
100 Assays |
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5 pmol |
Serum, cells, tissues |
100 Assays |
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20 pmol |
Cells, tissues |
100 Assays |
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100 pmol |
Cells, tissues |
100 Assays |
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100 pmol |
Cells, tissues |
100 Assays |
Enzyme
| Assay | Code | Read-out | Sensitivity | Sample Type | Pack Size | |||
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Cells, tissues |
100 Assays |
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Colorimetric/Fluorometric |
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Cells, tissues |
100 Assays |
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Colorimetric |
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Cells, tissues |
100 Assays |
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Fluorometric |
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100 Assays |
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Colorimetric |
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N/A |
100 Assays |
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Cells, tissues |
100 Assays |
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Serum, cells, tissues |
100 Assays |
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Cells, tissues |
100 Assays |
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4 mU |
Cells, tissues |
100 Assays |
Cofactors & Enzymes
| Assay | Code | Read-out | Sensitivity | Sample Type | Pack Size | ||||
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1000 pmol |
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100 Assays |
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Colorimetric/Fluorometric |
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100 Assays |
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100 Assays |
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100 Assays |
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100 Assays |
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100 Assays |
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10 pmol |
Serum, cells, tissues |
100 Assays |