Glycolysis Assay Kits


Glycolysis Assays

At Assay Genie we've developed a range of Glycolysis assays for researchers to analyse key steps in the glycolysis pathway.

Assay Genie's Glycolysis Assay Kit is based on measuring the production of L-Lactate from glycolysis in cells. L-Lactate that is secreted into the cell media when glycolysis occurs and is quantified using a coupled reaction involving the lactate dehydrogenase catalyzed oxidation of L-lactate that generates pyruvate and NADH which reduces a formazan dye.

The intensity of the reduced dye, measured at 565 nm, is directly proportional to the L-lactate concentration in the sample, which in turn is directly proportional to rate of glycolysis in the cells.

This assay can be used for the direct determination of L-Lactate produced by glycolysis in cell samples. Screening of glycolysis inhibitors.

Glycolysis Inhibition: PANC1 cells treated with varying concentrations of 2-deoxy-D-glucose in DMEM medium

Metabolites

Assay Code Read-out Sensitivity Sample Type Pack Size
BN00897

Fluorometric

50 pmol

Cells, tissues

100 Assays

BN00830

Colorimetric/Fluorometric

100 pmol

Serum, cells, tissues, urine

100 Assays

BN00910

Colorimetric

1000 pmol

Serum, cells, tissues, food

100 Assays

BN00912

Fluorometric

10 pmol

Serum, cells, tissues, food

100 Assays

BN00881

Colorimetric

1000 pmol

Serum, cells, tissues

100 Assays

BN00911
Fluorometric

5 pmol

Serum, cells, tissues

100 Assays

BN00991
Colorimetric/Fluorometric

20 pmol

Cells, tissues

100 Assays

BN00628
Colorimetric/Fluorometric

100 pmol

Cells, tissues

100 Assays

BN00833
Colorimetric/Fluorometric

100 pmol

Cells, tissues

100 Assays

Enzyme

Assay Code Read-out Sensitivity Sample Type Pack Size
BN00889
Colorimetric
100 mU

Cells, tissues

100 Assays

BN00915

Colorimetric/Fluorometric

0.04 mU

Cells, tissues

100 Assays

BN00904

Colorimetric

10 mU

Cells, tissues

100 Assays

BN00982

Fluorometric

2 μU
Serum, cells, tissues

100 Assays

BN00933

Colorimetric

N/A

N/A

100 Assays

BN00989
Colorimetric
1 mU

Cells, tissues

100 Assays

BN00988
Colorimetric
0.1 mU

Serum, cells, tissues

100 Assays

BN00929
Colorimetric/Fluorometric
1000 mU

Cells, tissues

100 Assays

BN00894
Colorimetric

4 mU

Cells, tissues

100 Assays

Cofactors & Enzymes

Assay Code Read-out Sensitivity Sample Type Pack Size
BN00619
Colorimetric

1000 pmol

Tissue, cells, Protein Prep.

100 Assays

BN00618

Colorimetric/Fluorometric

100 pmol
Tissue, cells, Protein Prep.

100 Assays

BN00522
Luminescence
<10 pmol
Cells, Protein Prep.

100 Assays

BN00617
Colorimetric/Fluorometric
100 pmol
Tissue, cells, Protein Prep.

100 Assays

BN00604
Fluorometric
<5 pmol
Tissue, cells, Protein Prep.

100 Assays

BN00603
Colorimetric
10 pmol
Tissue, cells, serum, urine

100 Assays

BN00611
Colorimetric

10 pmol

Serum, cells, tissues

100 Assays