Mouse Multiplex Cytokine ELISA Panel

The ELISA Genie, Mouse Cytokine multiplex assays utilizes multiple bead populations differentiated by size and different levels of fluorescence intensity. With multiple sizes of beads and multiple levels of fluorescence intensity in each bead size, the GeniePlex technology can measure up to 24 analytes simultaneously using as little as 15μL.

The bead populations in the reaction are determined by a flow cytometer equipped with either a single 488nm laser or dual 488nm and 633/640nm lasers. The maximum emission of the bead classification dye is at 700 nm.

Features & Benefits

  • Analyze: Multiplex 2-24 analytes in a single sample!
  • Fast: 2 hour protocol!
  • Sensitive: Measure as low as <10 pg/ml of each analyte
  • Dynamic: Lower limit < 20 pg/mL | Upper limit > 5,000 pg/mL
  • High Precision Intra-assay CV: < 10% | Inter-assay CV: < 20%
  • Low Volumes: Use as little as 15ul of sample
  • Validated: All assays fully tested for cross-reactivity in our lab

Data Analysis

Mouse Multiplex Cytokine ELISA Panels

Product Code Panel Name Number of Analytes Analytes

Mouse T Helper Cytokine 3-Plex Immunoassay Panel 1

3

IL-9, IL-10, and TNFα

Mouse T Helper Cytokine 3-Plex Immunoassay Panel 2

3

IFN-Gamma, IL-10, and TNF-Alpha

Mouse T Helper Cytokine 3-Plex Immunoassay Panel 3

3

IL-9, IL-10, and TNFα

Mouse T Helper Cytokine 4-Plex Immunoassay Panel 1

4

IFNγ, IL-4, IL-6, TNFα

Mouse T Helper Cytokine 4-Plex Immunoassay Panel 2

4

IFNγ, IL-4, IL-10, TNFα

Mouse T Helper Cytokine 4-Plex Immunoassay Panel 3

4

IL-6, IL-10, IL-22, TNFα

Mouse Th1/Th2 5-plex Immunoassay Panel

5

IFNγ, IL-2, IL-4, IL-5, TNFα

Mouse Th1/Th2 5-plex Immunoassay Panel

6

IFNγ, IL-2, IL-4, IL-6, IL-10, TNFα

Mouse Th1/Th2 5-plex Immunoassay Panel

6

IFNγ, IL-2, IL-4, IL-5, IL-10, TNFα

Mouse Th1/Th2 8-Plex Immunoassay Panel

8

IFNγ, IL-1α, IL-2, IL-4, IL-5, IL-6, IL-10, and TNFα

Mouse Th1/Th2/Th17 7-plex Kit

7

IFNγ, IL-2, IL-4, IL-6, IL-10, IL-17A, TNFα

Mouse Th1/Th2/Th17 7-plex Kit

10

GM-CSF, IFNγ, IL-1α, IL-2, IL-4, IL-5, IL-6, IL-10, IL-17A, TNFα

Mouse Th1/Th2/Th17 14-plex

14

IFNγ, IL-1α, IL-1β, IL-2 , IL-4, IL-5, IL-6, IL-10, IL-12p70, IL-13, IL-17A, IL-21, IL-22, TNFα

Mouse Th1/Th2/Th17 16-plex

16

GM-CSF, IFNγ, IL-1α, IL-1β, IL-2 , IL-4, IL-5, IL-6, IL-10, IL-12p70, IL-13, IL-17A, IL-21, IL-23p19, KC, TNFα

Mouse Th1/Th2/Th17 18-plex

18

GM-CSF, IFNγ, IL-1α, IL-1β, IL-2 , IL-4, IL-5, IL-6, IL-10, IL-12p70, IL-13, IL-17A, IL-21, IL-22, IL-23p19, KC, TNFα, TSLP

Mouse Inflammation 5-Plex Immunoassay Panel 1

5

IL-1β, IL-6, IL-10, MCP-1, TNFα

Genieplex Mouse Inflammation 5-Plex Immunoassay Panel 2

5

IL-6, IL-10, IL-12p70, MCP-1, TNFα

Mouse Inflammation 6-Plex Immunoassay Panel

6

IFNγ, IL-6, IL-10, IL-12p70, MCP-1, TNFα

Mouse Inflammation 7-Plex Immunoassay Panel 1

7

IFNγ, IL-1β, IL-6, IP-10, KC, MCP-1, TNFα

Mouse Inflammation 7-Plex Immunoassay Panel 2

7

IL-1α, IL-1β, IL-6, IL-10, IL-22, IL-23, TNFα

Mouse Inflammation 8-Plex Immunoassay Panel

8

IFNγ, IL-1α, IL-6, IL-10, IL-12p70, KC, MCP-1, TNFα

Mouse Inflammation 11-Plex

11

IFNγ, IL-1α, IL-1β, IL-6, IL-10, IL-12p70, IL-23p19, IP-10, KC, MCP-1, TNFα

Mouse Inflammation 17-Plex

17

IFNγ, IL-1α, IL-1β, IL-6, IL-9, IL-10, IL-12p70, IL-13, IL-15, IL-23p19, IP-10, KC, MCP-1, MIP-1α, MIP-1β, RANTES, TNFα

Mouse Chemokine 7-Plex Immunoassay Panel

7

Eotaxin, KC, MCP-1, MCP-3, MIG, RANTES, IP-10


Kit Contents

  • Analyte-Specific antibody conjugated beads
  • Analyte-Specific biotinylated detection antibodies
  • Lyophilized Antigen Standards (One Vial)
  • Antibody diluent
  • 1x Streptavidin-PE (SAPE)
  • 10x Reading Buffer
  • 10x Wash Buffer
  • PCR 8-tube Strip
  • Filter Plate and Lid
  • Plate Seals

Mouse Multiplex Cytokine ELISA Panel Video Protocol