GeniePlex Human IL-2RA/sCD25 Immunoassay
Product Name: GeniePlex Human IL-2RA/sCD25 Immunoassay
This GeniePlex Human IL-2RA/sCD25 Immunoassay is a bead-based Multiplex Immunoassay kit that enables the simultaneous and quantitative detection of IL-2RA/sCD25 on almost any flow cytometer in a wide variety of samples such as Serum, Plasma, Cell culture supernatant, Cell lysates, Tissue lysates and other samples types.
GeniePlex uses a unique mix of antibody-coated encoded microparticles providing an ultra-sensitive technology for the quantitation of analytes.
At ELISA Genie we understand the need for quantitative reproducible results! Therefore, we have developed a simplified protocol meaning you can easily quantify IL-2RA/sCD25 in as little as 15ul of sample all in 32-test or 96-test filter plate format for rapid analysis.
As our beads and standards come pre-mixed, simply add your pre-defined bead panel to the wells followed by the pre-mixed standards or samples. After an incubation and wash step, add Biotinylated detection antibodies and repeat incubation & wash. Next step is the addition of Streptavidin-PE which binds the Biotinylated detection antibody. After a final incubation & washing step, Reading buffer is added and the assay analysed on a flow cytometer with either a 488nm laser or 488nm and 633/640nm lasers. The concentration of the analytes in the samples is determined by comparing the fluorescent signals to a standard curve generated from a serial dilution of a known concentration of the analyte.
|1x Ab-conjugated beads (premixed)||S5P9|
|Sample types:||Cell culture supernatant, serum, plasma, bodily fluid and tissue/cell lysate|
|Sensitivity (LODs - pg/mL):||< 150 U/mL|
|LLOQ (pg/mL)||< 300 U/mL|
|ULOQ (pg/mL)||> 150,000 U/mL|
|Sample volume:||Sample volume: 15 µL/test|