GeniePlex Human PECAM-1/sCD31 Immunoassay

Product Name: GeniePlex Human PECAM-1/sCD31 Immunoassay

Analyte

PECAM-1/sCD31

Species Reactivity:

Human

Assay Type:

Multiplex

Detection Method:

Flow Cytometry

Assay Time:

2 hours

Product Overview

This GeniePlex Human PECAM-1/sCD31 Immunoassay is a bead-based Multiplex Immunoassay kit that enables the simultaneous and quantitative detection of PECAM-1/sCD31 on almost any flow cytometer in a wide variety of samples such as Serum, Plasma, Cell culture supernatant, Cell lysates, Tissue lysates and other samples types.

GeniePlex uses a unique mix of antibody-coated encoded microparticles providing an ultra-sensitive technology for the quantitation of analytes.

At ELISA Genie we understand the need for quantitative reproducible results! Therefore, we have developed a simplified protocol meaning you can easily quantify PECAM-1/sCD31 in as little as 15ul of sample all in 32-test or 96-test filter plate format for rapid analysis.

As our beads and standards come pre-mixed, simply add your pre-defined bead panel to the wells followed by the pre-mixed standards or samples. After an incubation and wash step, add Biotinylated detection antibodies and repeat incubation & wash. Next step is the addition of Streptavidin-PE which binds the Biotinylated detection antibody. After a final incubation & washing step, Reading buffer is added and the assay analysed on a flow cytometer with either a 488nm laser or 488nm and 633/640nm lasers. The concentration of the analytes in the samples is determined by comparing the fluorescent signals to a standard curve generated from a serial dilution of a known concentration of the analyte.

Product Information