Description
Product Name: GeniePlex Human PECAM-1/sCD31 Immunoassay
Analyte
PECAM-1/sCD31
Species Reactivity:
Human
Assay Type:
Multiplex
Detection Method:
Flow Cytometry
Assay Time:
2 hours
Product Overview
This GeniePlex Human PECAM-1/sCD31 Immunoassay is a bead-based Multiplex Immunoassay kit that enables the simultaneous and quantitative detection of PECAM-1/sCD31 on almost any flow cytometer in a wide variety of samples such as Serum, Plasma, Cell culture supernatant, Cell lysates, Tissue lysates and other samples types.
GeniePlex uses a unique mix of antibody-coated encoded microparticles providing an ultra-sensitive technology for the quantitation of analytes.
At ELISA Genie we understand the need for quantitative reproducible results! Therefore, we have developed a simplified protocol meaning you can easily quantify PECAM-1/sCD31 in as little as 15ul of sample all in 32-test or 96-test filter plate format for rapid analysis.
As our beads and standards come pre-mixed, simply add your pre-defined bead panel to the wells followed by the pre-mixed standards or samples. After an incubation and wash step, add Biotinylated detection antibodies and repeat incubation & wash. Next step is the addition of Streptavidin-PE which binds the Biotinylated detection antibody. After a final incubation & washing step, Reading buffer is added and the assay analysed on a flow cytometer with either a 488nm laser or 488nm and 633/640nm lasers. The concentration of the analytes in the samples is determined by comparing the fluorescent signals to a standard curve generated from a serial dilution of a known concentration of the analyte.
Product Information
Analytes | PECAM-1/sCD31 |
Reactivity | Human |
1x Ab-conjugated beads (premixed) | S4P3 |
Sample types: | Cell culture supernatant, serum, plasma, bodily fluid and tissue/cell lysate |
Sensitivity (LODs - pg/mL): | < 5 |
LLOQ (pg/mL) | < 10 |
ULOQ (pg/mL) | > 5,000 |
Sample volume: | Sample volume: 15 µL/test |
Additional Information
Product type: |
Multiplex |
Reactivity: |
Human |
ELISA Type: |
Multiplex |