Anti-ARHGEF9 Antibody (CAB7964)

Antibody Name:	ARHGEF9 Rabbit Polyclonal Antibody
Antibody SKU:	CAB7964
Antibody Size:	20uL, 50uL, 100uL
Application:	WB IHC IF
Reactivity:	Human, Mouse, Rat
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 307-516 of human ARHGEF9 (NP_056000.1).

Application:	WB IHC IF
Recommended Dilution:	WB 1:500 - 1:2000 IHC 1:50 - 1:200 IF 1:50 - 1:100
Reactivity:	Human, Mouse, Rat
Positive Samples:	BT-474, 22Rv1, HeLa

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 307-516 of human ARHGEF9 (NP_056000.1).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	WEGE DILD RSSE LIYT GEMA WIYQ PYGR NQQR VFFL FDHQ MVLC KKDL IRRD ILYY KGRI DMDK YEVV DIED GRDD DFNV SMKN AFKL HNKE TEEI HLFF AKKL EEKI RWLR AFRE ERKM VQED EKIG FEIS ENQK RQAA MTVR KVPK QKGV NSAR SVPP SYPP PQDP LNHG QYLV PDGI AQSQ VFEF TEPK RSQS PFWQ NFSR LTPF KK
Gene ID:	23229
Uniprot:	O43307
Cellular Location:	Cytoplasm
Calculated MW:	49kDa/54kDa/60kDa
Observed MW:	61kDa

Synonyms:	ARHGEF9, COLLYBISTIN, EIEE8, HPEM-2, PEM-2, PEM2
Background:	The protein encoded by this gene is a Rho-like GTPase that switches between the active (GTP-bound) state and inactive (GDP-bound) state to regulate CDC42 and other genes. Defects in this gene are a cause of startle disease with epilepsy (STHEE), also known as hyperekplexia with epilepsy. Three transcript variants encoding different isoforms have been found for this gene.

UniProt Protein Function:	ARHGEF9: Acts as guanine nucleotide exchange factor (GEF) for CDC42. Promotes formation of GPHN clusters. Defects in ARHGEF9 are the cause of pileptic encephalopathy, early infantile, type 8 (EIEE8). A disorder characterized by hyperekplexia and early infantile epileptic encephalopathy. Neurologic features include exaggerated startle response, seizures, impaired psychomotor development, and mental retardation. Seizures can be provoked by tactile stimulation or extreme emotion. 2 isoforms of the human protein are produced by alternative splicing.
UniProt Protein Details:	Protein type:GAPs; GAPs, Rac/Rho Chromosomal Location of Human Ortholog: Xq11.1 Cellular Component: cytoplasm; cytosol Molecular Function:guanyl-nucleotide exchange factor activity; Rho guanyl-nucleotide exchange factor activity Biological Process: positive regulation of apoptosis; regulation of small GTPase mediated signal transduction Disease: Epileptic Encephalopathy, Early Infantile, 8
NCBI Summary:	The protein encoded by this gene is a Rho-like GTPase that switches between the active (GTP-bound) state and inactive (GDP-bound) state to regulate CDC42 and other genes. Defects in this gene are a cause of startle disease with epilepsy (STHEE), also known as hyperekplexia with epilepsy. Three transcript variants encoding different isoforms have been found for this gene.[provided by RefSeq, Mar 2010]
UniProt Code:	O43307
NCBI GenInfo Identifier:	111599692
NCBI Gene ID:	23229
NCBI Accession:	BC117406
UniProt Secondary Accession:	O43307,Q5JSL6, A8K1S8, B4DHC7, F8W7P8,
UniProt Related Accession:	O43307
Molecular Weight:	49,458 Da
NCBI Full Name:	Homo sapiens Cdc42 guanine nucleotide exchange factor (GEF) 9, mRNA
NCBI Synonym Full Names:	Cdc42 guanine nucleotide exchange factor 9
NCBI Official Symbol:	ARHGEF9
NCBI Official Synonym Symbols:	PEM2; EIEE8; PEM-2; HPEM-2; COLLYBISTIN
NCBI Protein Information:	rho guanine nucleotide exchange factor 9
UniProt Protein Name:	Rho guanine nucleotide exchange factor 9
UniProt Synonym Protein Names:	Collybistin; PEM-2 homolog; Rac/Cdc42 guanine nucleotide exchange factor 9
Protein Family:	Rho guanine nucleotide exchange factor
UniProt Gene Name:	ARHGEF9
UniProt Entry Name:	ARHG9_HUMAN

	Western blot analysis of extracts of various cell lines, using ARHGEF9 antibody (CAB7964) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 90s.
	Immunohistochemistry of paraffin-embedded mouse heart using ARHGEF9 antibody (CAB7964) at dilution of 1:100 (40x lens).
	Immunofluorescence analysis of A549 cells using ARHGEF9 antibody (CAB7964). Blue: DAPI for nuclear staining.

Antibodies

ARHGEF9 Antibody (PACO03692)

Secondary Antibody

Anti-HRP Goat Anti-Rabbit IgG (H+L) Antibody (CABS014)