Anti-USP4 Antibody (CAB20005)[KO Validated]

SKU:
CAB20005
€129 - €349

Description

Technical Manual

Anti-USP4 Antibody [KO Validated]

Product Name:Anti-USP4 Antibody [KO Validated]
Product SKU:CAB20005
Size:20uL, 50 uL
Host Species:Rabbit
Purification Method:Affinity purification
Isotype:IgG
Background:

The protein encoded by this gene is a protease that deubiquitinates target proteins such as ADORA2A and TRIM21. The encoded protein shuttles between the nucleus and cytoplasm and is involved in maintaining operational fidelity in the endoplasmic reticulum. Three transcript variants encoding different isoforms have been found for this gene.

Immunogen:Recombinant protein of human USP4.
Sequence:

Email for sequence

Gene ID:7375
Swiss Prot:Q13107
Synonyms:USP4; UNP; Unph
Calculated MW:35kDa/103kDa/108kDa
Observed MW:108KDa
Reactivity:Human, Mouse, Rat
Application:WB IHC
Recommended Dilution:WB 1:500 - 1:2000 IHC 1:50 - 1:200
Storage Buffer:Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Application Key:Western blotting Immunohistochemistry
Positive Samples:HeLa
Cellular Location:Cytoplasm, Nucleus

Product Images

Anti-USP4 Antibody [KO Validated] (CAB20005)

Western blot analysis of extracts from normal (control) and USP4 knockout (KO) HeLa cells, using USP4 antibody (CAB20005) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 1s.

Uniprot Information

UniProt Protein Function:USP4: Hydrolase that deubiquitinates target proteins such as the receptor ADORA2A, PDPK1 and TRIM21. Deubiquitination of ADORA2A increases the amount of functional receptor at the cell surface. Plays a role in the regulation of quality control in the ER. Interacts with RB1 (both dephosphorylated and hypophosphorylated forms). Interacts with ADORA2A (via cytoplasmic C-terminus); the interaction is direct. Interacts with RB1, RBL1 and RBL2. Overexpressed in small cell tumors and adenocarcinomas of the lung compared to wild-type lung. Expressed in the hippocampal neurons. Belongs to the peptidase C19 family. USP4 subfamily. 2 isoforms of the human protein are produced by alternative splicing.
UniProt Protein Details:

Protein type:EC 3.4.19.12; Oncoprotein; Protease; Ubiquitin conjugating system; Ubiquitin-specific protease

Chromosomal Location of Human Ortholog: 3p21.31

Cellular Component: cytoplasm; cytosol; lysosome; nucleus; plasma membrane

Molecular Function:adenosine receptor binding; identical protein binding; protein binding; ubiquitin-specific protease activity

Biological Process: assembly of spliceosomal tri-snRNP; negative regulation of protein ubiquitination; protein deubiquitination; regulation of protein stability

NCBI Summary:The protein encoded by this gene is a protease that deubiquitinates target proteins such as ADORA2A and TRIM21. The encoded protein shuttles between the nucleus and cytoplasm and is involved in maintaining operational fidelity in the endoplasmic reticulum. Three transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Oct 2011]
UniProt Code:Q13107
NCBI GenInfo Identifier:116242839
NCBI Gene ID:7375
NCBI Accession:Q13107.3
UniProt Secondary Accession:Q13107,O43452, O43453, Q08AK8, A8K6Y0, C9IY91,
UniProt Related Accession:Q13107
Molecular Weight:109kDa
NCBI Full Name:Ubiquitin carboxyl-terminal hydrolase 4
NCBI Synonym Full Names:ubiquitin specific peptidase 4
NCBI Official Symbol:USP4
NCBI Official Synonym Symbols:UNP; Unph
NCBI Protein Information:ubiquitin carboxyl-terminal hydrolase 4
UniProt Protein Name:Ubiquitin carboxyl-terminal hydrolase 4
UniProt Synonym Protein Names:Deubiquitinating enzyme 4; Ubiquitin thioesterase 4; Ubiquitin-specific-processing protease 4; Ubiquitous nuclear protein homolog
UniProt Gene Name:USP4
View AllClose

Additional Information

Product type:
Antibody
Reactivity:
Human
Reactivity:
Mouse
Reactivity:
Rat
Host Species:
Rabbit
Isotype:
IgG
Research Area:
Signal Transduction
View AllClose