FQNs (Fluoroquinolones) ELISA Kit (FSES0037)

SKU:
FSES0037
€377

Description

Technical Manual

FQNs (Fluoroquinolones) ELISA Kit

Kit Name: FQNs (Fluoroquinolones) ELISA Kit
Kit Size: 96 Assays
Test Principle: This kit uses Competitive-ELISA as the method. It can detect Fluoroquinolones/Quinolones (FQNS) in samples, such as milk, muscle, honey, etc. This kit is composed of ELISA Microtiter plate, HRP conjugate, antibody working solution, standard and other supplementary reagents. The microtiter plate in this kit has been pre-coated with coupled antigen. During the reaction, FQNS in the samples or standard competes with coupled antigen on the solid phase supporter for sites of anti-FQNS antibody. Then Horseradish Peroxidase (HRP) conjugate is added to each microtiter plate well, and substrate reagent is added for color development. There is a negative correlation between the OD value of samples and the concentration of FQNS. The concentration of FQNS in the samples can be calculated by comparing the OD of the samples to the standard curve.
Sensitivity: 0.2 ppb (ng/mL)
Reaction Mode: 25°C,45 min~15min
Detection Limit: Muscle ---0.6 ppb; Honey ---0.8 ppb; Milk---6 ppb; Milk powder---12 ppb; Eggs---6 ppb; Urine---1 ppb.
Cross Reactivity:

Enrofloxacin

100%

Norfloxacin

174%

Ciprofloxacin

170%

Lomefloxacin

132%

Flumequine

126%

Peflacine

125%

Danofloxacin

110%

Sarafloxacin

107%

Difloxacin

84%

Enoxacin

66%

Ofloxacin (racemate)

58%

Oxolinic acid

28%

Levofloxacin

10%

Marbofloxacin

4%

Sample Recovery Rate: Muscle, Honey, Milk, Milk powder, Eggs, Urine ---85%±15%.
Kits Components:
Enrofloxacin 100%
Norfloxacin 174%
Ciprofloxacin 170%
Lomefloxacin 132%
Flumequine 126%
Peflacine 125%
Danofloxacin 110%
Sarafloxacin 107%
Difloxacin 84%
Enoxacin 66%
Ofloxacin (racemate) 58%
Oxolinic acid 28%
Levofloxacin 10%
Marbofloxacin 4%
Instruments: Microplate reader, Printer, Homogenizer, Oscillators, Nitrogen evaporators, Water bath, Centrifuge, Graduated pipette, Balance (sensibility 0.01g).
Micropipette: Single channel (20-200 uL, 100-1000 uL), Multichannel (30-300 uL).
Reagents: Anhydrous acetonitrile, N-hexane, Concentrated HCl, Dichloromethane
Storage And Expiry Date: Store the kit at 2~8°C. Do not freeze any test kit components. Return any unused microwells to their original foil bag and reseal them together with the desiccant provided and further store at 2 - 8°C. Expiry date: expiration date is on the packing box.
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Additional Information

Product type:
ELISA
Application:
ELISA
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