Description
Description | Sialic-acid-binding immunoglobulin-like lectin (Siglec) that plays a role in mediating cell-cell interactions and in maintaining immune cells in a resting state. Preferentially recognizes and binds alpha-2,3- and more avidly alpha-2,6-linked sialic acid-bearing glycans. Upon engagement of ligands such as C1q or syalylated glycoproteins, two immunoreceptor tyrosine-based inhibitory motifs (ITIMs) located in CD33 cytoplasmic tail are phosphorylated by Src-like kinases such as LCK. These phosphorylations provide docking sites for the recruitment and activation of protein-tyrosine phosphatases PTPN6/SHP-1 and PTPN11/SHP-2. In turn, these phosphatases regulate downstream pathways through dephosphorylation of signaling molecules. One of the repressive effect of CD33 on monocyte activation requires phosphoinositide 3-kinase/PI3K. |
Product name | Anti-CD33 antibody (DM77) Rabbit mAb |
Synonyms | CD33,SIGLEC3,gp67 |
Type | Primary |
Quantity | 100 µL |
Shipping | Blue Ice |
Antigen | CD33 |
Reactivity | Human |
Host | Rabbit |
Clonality | Monoclonal |
Clone | DM77 |
Isotype | Rabbit IgG |
Application | ELISA,Flow Cyt |
Immunogen substitute | Recombinant human CD33 (Asp18-His269) (HDPT0037) produced by using human HEK293 cells |
Purification | Purified from cell culture supernatant by affinity chromatography |
Format | Liquid |
Storage | Store at -20°C for 12 months (Avoid repeated freezing and thawing) |
Usage | Research use only |
Buffer | 50% Glycerol; PBS, pH 7.4; 0.1% BSA |
Preservative | 0.1% Procline 300 |
Reconstitution | Reconstitute with deionized water |
Application Advice | ELISA 1/5000-10000;Flow Cyt 1/100 |
Gene ID | 945 |
Swiss-Prot | P20138 |
Molecular Weight | 39.8kDa |
Assay Genie Related SKUs | HDPT0037 |