Anti-ASF1A Antibody (CAB6528)

Antibody Name:	Anti-ASF1A Antibody
Antibody SKU:	CAB6528
Antibody Size:	20uL, 50uL, 100uL
Application:	WB IF
Reactivity:	Human, Mouse
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 1-204 of human ASF1A (NP_054753.1).

Application:	WB IF
Recommended Dilution:	WB 1:500 - 1:2000 IF 1:50 - 1:100
Reactivity:	Human, Mouse
Positive Samples:	Mouse pancreas

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 1-204 of human ASF1A (NP_054753.1).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	MAKV QVNN VVVL DNPS PFYN PFQF EITF ECIE DLSE DLEW KIIY VGSA ESEE YDQV LDSV LVGP VPAG RHMF VFQA DAPN PGLI PDAD AVGV TVVL ITCT YRGQ EFIR VGYY VNNE YTET ELRE NPPV KPDF SKLQ RNIL ASNP RVTR FHIN WEDN TEKL EDAE SSNP NLQS LLST DALP SASK GWST SENS LNVM LESH MDCM
Gene ID:	25842
Uniprot:	Q9Y294
Cellular Location:	Nucleus
Calculated MW:	22kDa
Observed MW:	23kDa

Synonyms:	ASF1A, CGI-98, CIA, HSPC146
Background:	This gene encodes a member of the H3/H4 family of histone chaperone proteins and is similar to the anti-silencing function-1 gene in yeast. The protein is a key component of a histone donor complex that functions in nucleosome assembly. It interacts with histones H3 and H4, and functions together with a chromatin assembly factor during DNA replication and repair.

UniProt Protein Function:	ASF1A: Histone chaperone that facilitates histone deposition and histone exchange and removal during nucleosome assembly and disassembly. Cooperates with chromatin assembly factor 1 (CAF-1) to promote replication-dependent chromatin assembly and with HIRA to promote replication-independent chromatin assembly. Required for the formation of senescence-associated heterochromatin foci (SAHF) and efficient senescence-associated cell cycle exit. Interacts with histone H3 (including both histone H3.1 and H3.3) and histone H4. Interacts with the CHAF1A, CHAF1B and RBBP4 subunits of the CAF-1 complex. Interacts with CABIN1, HAT1, HIRA, NASP, TAF1, TLK1, TLK2 and UBN1. Ubiquitously expressed. Belongs to the ASF1 family.
UniProt Protein Details:	Protein type:DNA repair, damage; Histone-binding Chromosomal Location of Human Ortholog: 6q22.31 Cellular Component: nuclear chromatin; nucleoplasm; nucleus; protein complex Molecular Function:chromatin binding; histone binding; protein binding Biological Process: chromatin modification; DNA repair; DNA replication-dependent nucleosome assembly; DNA replication-independent nucleosome assembly; muscle cell differentiation; negative regulation of chromatin silencing; nucleosome assembly; osteoblast differentiation; transcription, DNA-dependent
NCBI Summary:	This gene encodes a member of the H3/H4 family of histone chaperone proteins and is similar to the anti-silencing function-1 gene in yeast. The protein is a key component of a histone donor complex that functions in nucleosome assembly. It interacts with histones H3 and H4, and functions together with a chromatin assembly factor during DNA replication and repair. [provided by RefSeq, Jul 2008]
UniProt Code:	Q9Y294
NCBI GenInfo Identifier:	74735206
NCBI Gene ID:	25842
NCBI Accession:	Q9Y294.1
UniProt Secondary Accession:	Q9Y294,Q6IA08, Q9P014,
UniProt Related Accession:	Q9Y294
Molecular Weight:	22,969 Da
NCBI Full Name:	Histone chaperone ASF1A
NCBI Synonym Full Names:	anti-silencing function 1A histone chaperone
NCBI Official Symbol:	ASF1A
NCBI Official Synonym Symbols:	CIA; CGI-98; HSPC146
NCBI Protein Information:	histone chaperone ASF1A
UniProt Protein Name:	Histone chaperone ASF1A
UniProt Synonym Protein Names:	Anti-silencing function protein 1 homolog A; hAsf1; hAsf1a; CCG1-interacting factor A; CIA; hCIA
Protein Family:	Histone chaperone
UniProt Gene Name:	ASF1A
UniProt Entry Name:	ASF1A_HUMAN

	Western blot analysis of extracts of mouse pancreas, using ASF1A Antibody (CAB6528) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit (RM00021). Exposure time: 20s.
	Immunofluorescence analysis of HeLa cells using ASF1A antibody (CAB6528). Blue: DAPI for nuclear staining.

Antibodies

Anti-ASF1A Antibody (CAB14508)

Anti-ASF1A Antibody (CAB17124)

ASF1A Antibody (PACO00434)

ASF1A Antibody (PACO07880)

Secondary Antibody

Anti-HRP Goat Anti-Rabbit IgG (H+L) Antibody (CABS014)