Anti-BAMBI Antibody (CAB6532)

Antibody Name:	Anti-BAMBI Antibody
Antibody SKU:	CAB6532
Antibody Size:	20uL, 50uL, 100uL
Application:	WB IF
Reactivity:	Human, Rat
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 21-152 of human BAMBI (NP_036474.1).

Application:	WB IF
Recommended Dilution:	WB 1:500 - 1:2000 IF 1:50 - 1:100
Reactivity:	Human, Rat
Positive Samples:	A-549, Rat heart

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 21-152 of human BAMBI (NP_036474.1).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	VLLT KGEI RCYC DAAH CVAT GYMC KSEL SACF SRLL DPQN SNSP LTHG CLDS LAST TDIC QAKQ ARNH SGTT IPTL ECCH EDMC NYRG LHDV LSPP RGEA SGQG NRYQ HDGS RNLI TKVQ ELTS SKEL WFRA
Gene ID:	25805
Uniprot:	Q13145
Cellular Location:	Membrane, Single-pass type I membrane protein
Calculated MW:	29kDa
Observed MW:	55kDa

Synonyms:

BAMBI, NMA

Background:

This gene encodes a transmembrane glycoprotein related to the type I receptors of the transforming growth factor-beta (TGF-beta) family, whose members play important roles in signal transduction in many developmental and pathological processes. The encoded protein however is a pseudoreceptor, lacking an intracellular serine/threonine kinase domain required for signaling. Similar proteins in frog, mouse and zebrafish function as negative regulators of TGF-beta, which has led to the suggestion that the encoded protein may function to limit the signaling range of the TGF-beta family during early embryogenesis.

UniProt Protein Function:	BAMBI: Negatively regulates TGF-beta signaling. Belongs to the BAMBI family.
UniProt Protein Details:	Protein type:Membrane protein, integral; Inhibitor Chromosomal Location of Human Ortholog: 10p12.3-p11.2 Cellular Component: cytoplasm; integral to membrane; plasma membrane Molecular Function:frizzled binding; punt binding Biological Process: regulation of cell shape; cell migration; positive regulation of protein binding; positive regulation of transcription, DNA-dependent; transforming growth factor beta receptor signaling pathway; positive regulation of cell proliferation; negative regulation of transforming growth factor beta receptor signaling pathway
NCBI Summary:	This gene encodes a transmembrane glycoprotein related to the type I receptors of the transforming growth factor-beta (TGF-beta) family, whose members play important roles in signal transduction in many developmental and pathological processes. The encoded protein however is a pseudoreceptor, lacking an intracellular serine/threonine kinase domain required for signaling. Similar proteins in frog, mouse and zebrafish function as negative regulators of TGF-beta, which has led to the suggestion that the encoded protein may function to limit the signaling range of the TGF-beta family during early embryogenesis. [provided by RefSeq, Jul 2008]
UniProt Code:	Q13145
NCBI GenInfo Identifier:	2498645
NCBI Gene ID:	25805
NCBI Accession:	Q13145.1
UniProt Related Accession:	Q13145
Molecular Weight:	260
NCBI Full Name:	BMP and activin membrane-bound inhibitor homolog
NCBI Synonym Full Names:	BMP and activin membrane-bound inhibitor
NCBI Official Symbol:	BAMBI
NCBI Official Synonym Symbols:	NMA
NCBI Protein Information:	BMP and activin membrane-bound inhibitor homolog; non-metastatic gene A protein; putative transmembrane protein NMA
UniProt Protein Name:	BMP and activin membrane-bound inhibitor homolog
UniProt Synonym Protein Names:	Non-metastatic gene A protein; Putative transmembrane protein NMA
Protein Family:	BMP and activin membrane-bound inhibitor
UniProt Gene Name:	BAMBI
UniProt Entry Name:	BAMBI_HUMAN

	Western blot analysis of extracts of various cell lines, using BAMBI antibody (CAB6532) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 10s.
	Immunofluorescence analysis of HeLa cells using BAMBI antibody (CAB6532). Blue: DAPI for nuclear staining.

Antibodies

Anti-BAMBI Antibody (CAB13589)

BAMBI Antibody (PACO07977)

Secondary Antibody

Anti-HRP Goat Anti-Rabbit IgG (H+L) Antibody (CABS014)