Anti-MRPL15 Antibody (CAB14400)

Antibody Name:	Anti-MRPL15 Antibody
Antibody SKU:	CAB14400
Antibody Size:	20uL, 50uL, 100uL
Application:	WB
Reactivity:	Human, Mouse
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 1-296 of human MRPL15 (NP_054894.1).

Application:	WB
Recommended Dilution:	WB 1:500 - 1:2000
Reactivity:	Human, Mouse
Positive Samples:	U-87MG, HeLa, MCF7, A-549, Mouse heart, Mouse kidney, Mouse liver

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 1-296 of human MRPL15 (NP_054894.1).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	MAGP LQGG GARA LDLL RGLP RVSL ANLK PNPG SKKP ERRP RGRR RGRK CGRG HKGE RQRG TRPR LGFE GGQT PFYI RIPK YGFN EGHS FRRQ YKPL SLNR LQYL IDLG RVDP SQPI DLTQ LVNG RGVT IQPL KRDY GVQL VEEG ADTF TAKV NIEV QLAS ELAI AAIE KNGG VVTT AFYD PRSL DIVC KPVP FFLR GQPI PKRM LPPE ELVP YYTD AKNR GYLA DPAK FPEA RLEL ARKY GYIL PDIT KDEL FKML CTRK DPRQ IFFG LAPG WVVN MADK KILK PTDE NLLK YYTS
Gene ID:	29088
Uniprot:	Q9P015
Cellular Location:	Mitochondrion
Calculated MW:	33kDa
Observed MW:	36kDa

Synonyms:

MRPL15, HSPC145, L15mt, MRP-L15, MRP-L7, RPML7

Background:

Mammalian mitochondrial ribosomal proteins are encoded by nuclear genes and help in protein synthesis within the mitochondrion. Mitochondrial ribosomes (mitoribosomes) consist of a small 28S subunit and a large 39S subunit. They have an estimated 75% protein to rRNA composition compared to prokaryotic ribosomes, where this ratio is reversed. Another difference between mammalian mitoribosomes and prokaryotic ribosomes is that the latter contain a 5S rRNA. Among different species, the proteins comprising the mitoribosome differ greatly in sequence, and sometimes in biochemical properties, which prevents easy recognition by sequence homology. This gene encodes a 39S subunit protein that belongs to the EcoL15 ribosomal protein family. A pseudogene corresponding to this gene is found on chromosome 15q.

UniProt Protein Function:	MRPL15: a mitochondrial ribosomal protein encoded by a nuclear gene that helps in protein synthesis within the mitochondrion. Mitochondrial ribosomes (mitoribosomes) consist of a small 28S subunit and a large 39S subunit. They have an estimated 75% protein to rRNA composition compared to prokaryotic ribosomes, where this ratio is reversed. Another difference between mammalian mitoribosomes and prokaryotic ribosomes is that the latter contain a 5S rRNA. Among different species, the proteins comprising the mitoribosome differ greatly in sequence, and sometimes in biochemical properties, which prevents easy recognition by sequence homology. This gene encodes a 39S subunit protein that belongs to the EcoL15 ribosomal protein family. A pseudogene corresponding to this gene is found on chromosome 15q. [provided by RefSeq, Jul 2008]
UniProt Protein Details:	Protein type:Translation; Mitochondrial; Ribosomal; RNA-binding Chromosomal Location of Human Ortholog: 8q11.2-q13 Cellular Component: mitochondrion; mitochondrial inner membrane; mitochondrial large ribosomal subunit Molecular Function:structural constituent of ribosome Biological Process: mitochondrial translation; organelle organization and biogenesis
NCBI Summary:	Mammalian mitochondrial ribosomal proteins are encoded by nuclear genes and help in protein synthesis within the mitochondrion. Mitochondrial ribosomes (mitoribosomes) consist of a small 28S subunit and a large 39S subunit. They have an estimated 75% protein to rRNA composition compared to prokaryotic ribosomes, where this ratio is reversed. Another difference between mammalian mitoribosomes and prokaryotic ribosomes is that the latter contain a 5S rRNA. Among different species, the proteins comprising the mitoribosome differ greatly in sequence, and sometimes in biochemical properties, which prevents easy recognition by sequence homology. This gene encodes a 39S subunit protein that belongs to the EcoL15 ribosomal protein family. A pseudogene corresponding to this gene is found on chromosome 15q. [provided by RefSeq, Jul 2008]
UniProt Code:	Q9P015
NCBI GenInfo Identifier:	7661806
NCBI Gene ID:	29088
NCBI Accession:	NP_054894.1
UniProt Related Accession:	Q9P015
Molecular Weight:	33kDa
NCBI Full Name:	39S ribosomal protein L15, mitochondrial
NCBI Synonym Full Names:	mitochondrial ribosomal protein L15
NCBI Official Symbol:	MRPL15
NCBI Official Synonym Symbols:	L15mt; RPML7; MRP-L7; HSPC145; MRP-L15
NCBI Protein Information:	39S ribosomal protein L15, mitochondrial
UniProt Protein Name:	39S ribosomal protein L15, mitochondrial
Protein Family:	39S ribosomal protein
UniProt Gene Name:	MRPL15
UniProt Entry Name:	RM15_HUMAN

Western blot analysis of extracts of various cell lines, using MRPL15 antibody (CAB14400) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 90s.

Antibodies

MRPL15 Antibody (PACO01092)

MRPL15 Antibody (PACO10576)

Secondary Antibody

Anti-HRP Goat Anti-Rabbit IgG (H+L) Antibody (CABS014)