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Anti-MDM4 Antibody (CAB1747)

SKU:
CAB1747
Product Type:
Antibody
Reactivity:
Human
Host Species:
Rabbit
Isotype:
IgG
Antibody Type:
Polyclonal Antibody
Research Area:
Cell Biology
€139 - €419
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Description

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Antibody Name:Anti-MDM4 Antibody
Antibody SKU:CAB1747
Antibody Size:100uL
Application:WB
Reactivity:Human
Host Species:Rabbit
Immunogen:A synthetic peptide of human MDM4
Application:WB
Recommended Dilution:WB 1:500 - 1:1000
Reactivity:Human
Positive Samples:ZR-75-1
Immunogen:A synthetic peptide of human MDM4
Purification Method:Affinity purification
Storage Buffer:Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:IgG
Sequence:Email for sequence
Gene ID:4194
Uniprot:O15151
Cellular Location:Nucleus
Calculated MW:13kDa/14kDa/18kDa/49kDa/54kDa
Observed MW:47kDa
Synonyms:MDM4, HDMX, MDMX, MRP1
Background:This gene encodes a nuclear protein that contains a p53 binding domain at the N-terminus and a RING finger domain at the C-terminus, and shows structural similarity to p53-binding protein MDM2. Both proteins bind the p53 tumor suppressor protein and inhibit its activity, and have been shown to be overexpressed in a variety of human cancers. However, unlike MDM2 which degrades p53, this protein inhibits p53 by binding its transcriptional activation domain. This protein also interacts with MDM2 protein via the RING finger domain, and inhibits the latter's degradation. So this protein can reverse MDM2-targeted degradation of p53, while maintaining suppression of p53 transactivation and apoptotic functions. Alternatively spliced transcript variants encoding different isoforms have been noted for this gene.
UniProt Protein Function:MDM4: Inhibits p53/TP53- and TP73/p73-mediated cell cycle arrest and apoptosis by binding its transcriptional activation domain. Inhibits degradation of MDM2. Can reverse MDM2-targeted degradation of TP53 while maintaining suppression of TP53 transactivation and apoptotic functions. Interacts with MDM2, TP53, TP73 and USP2. Found in a trimeric complex with UPB2, MDM2 and MDM4. Interacts (phosphorylated) with YWHAG; negatively regulates MDM4 activity toward TP53. Down-regulated by cisplatin. Expressed in all tissues tested with high levels in thymus. Belongs to the MDM2/MDM4 family. 5 isoforms of the human protein are produced by alternative splicing.
UniProt Protein Details:

Protein type:Ubiquitin ligase; Ligase; Ubiquitin conjugating system; EC 6.3.2.-

Chromosomal Location of Human Ortholog: 1q32

Cellular Component: nucleoplasm; nucleus

Molecular Function:enzyme binding; protein binding; zinc ion binding

Biological Process: cell proliferation; DNA damage response, signal transduction by p53 class mediator; DNA damage response, signal transduction by p53 class mediator resulting in cell cycle arrest; G0 to G1 transition; negative regulation of cell proliferation; negative regulation of protein catabolic process; negative regulation of transcription from RNA polymerase II promoter; protein complex assembly; protein stabilization

NCBI Summary:This gene encodes a nuclear protein that contains a p53 binding domain at the N-terminus and a RING finger domain at the C-terminus, and shows structural similarity to p53-binding protein MDM2. Both proteins bind the p53 tumor suppressor protein and inhibit its activity, and have been shown to be overexpressed in a variety of human cancers. However, unlike MDM2 which degrades p53, this protein inhibits p53 by binding its transcriptional activation domain. This protein also interacts with MDM2 protein via the RING finger domain, and inhibits the latter's degradation. So this protein can reverse MDM2-targeted degradation of p53, while maintaining suppression of p53 transactivation and apoptotic functions. Alternatively spliced transcript variants encoding different isoforms have been noted for this gene. [provided by RefSeq, Feb 2011]
UniProt Code:O15151
NCBI GenInfo Identifier:76803799
NCBI Gene ID:4194
NCBI Accession:O15151.2
UniProt Secondary Accession:O15151,Q2M2Y2, Q32SL2, Q6GS18, Q8IV83,
UniProt Related Accession:O15151
Molecular Weight:49,541 Da
NCBI Full Name:Protein Mdm4
NCBI Synonym Full Names:MDM4, p53 regulator
NCBI Official Symbol:MDM4  
NCBI Official Synonym Symbols:HDMX; MDMX; MRP1  
NCBI Protein Information:protein Mdm4
UniProt Protein Name:Protein Mdm4
UniProt Synonym Protein Names:Double minute 4 protein; Mdm2-like p53-binding protein; Protein Mdmx; p53-binding protein Mdm4
Protein Family:Protein
UniProt Gene Name:MDM4  
UniProt Entry Name:MDM4_HUMAN
Anti-MDM4 Antibody (CAB1747)Western blot analysis of extracts of ZR-75-1 cells, using MDM4 antibody (CAB1747). Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST.

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