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Anti-Villin-1 Antibody (CAB4691)

SKU:
CAB4691
Product Type:
Antibody
Reactivity:
Human
Host Species:
Rabbit
Isotype:
IgG
Research Area:
Cell Death
€139 - €419
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Description

system_update_altDatasheetsystem_update_altMSDS
Antibody Name:Anti-Villin-1 Antibody
Antibody SKU:CAB4691
Antibody Size:20uL, 50uL, 100uL
Application:WB
Reactivity:Human
Host Species:Rabbit
Immunogen:A synthesized peptide derived from human Villin-1
Application:WB
Recommended Dilution:WB 1:500 - 1:2000
Reactivity:Human
Positive Samples:HepG2
Immunogen:A synthesized peptide derived from human Villin-1
Purification Method:Affinity purification
Storage Buffer:Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 0.05% BSA, 50% glycerol, pH7.3.
Isotype:IgG
Sequence:Email for sequence
Gene ID:7429
Uniprot:P09327
Cellular Location:
Calculated MW:93kDa
Observed MW:95KDa
Synonyms:D2S1471, VIL
Background:This gene encodes a member of a family of calcium-regulated actin-binding proteins. This protein represents a dominant part of the brush border cytoskeleton which functions in the capping, severing, and bundling of actin filaments. Two mRNAs of 2.7 kb and 3.5 kb have been observed; they result from utilization of alternate poly-adenylation signals present in the terminal exon. [provided by RefSeq, Jul 2008]
UniProt Protein Function:Villin: Epithelial cell-specific Ca(2+)-regulated actin- modifying protein that modulates the reorganization of microvillar actin filaments. Plays a role in the actin nucleation, actin filament bundle assembly, actin filament capping and severing. Binds phosphatidylinositol 4,5-bisphosphate (PIP2) and lysophosphatidic acid (LPA); binds LPA with higher affinity than PIP2. Binding to LPA increases its phosphorylation by SRC and inhibits all actin-modifying activities. Binding to PIP2 inhibits actin-capping and -severing activities but enhances actin-bundling activity. Regulates the intestinal epithelial cell morphology, cell invasion, cell migration and apoptosis. Protects against apoptosis induced by dextran sodium sulfate (DSS) in the gastrointestinal epithelium. Appears to regulate cell death by maintaining mitochondrial integrity. Enhances hepatocyte growth factor (HGF)-induced epithelial cell motility, chemotaxis and wound repair. Upon S.flexneri cell infection, its actin-severing activity enhances actin-based motility of the bacteria and plays a role during the dissemination. Monomer. Homodimer; homodimerization is necessary for actin-bundling. Associates with F-actin; phosphorylation at tyrosines residues decreases the association with F-actin. Interacts (phosphorylated at C-terminus tyrosine phosphorylation sites) with PLCG1 (via the SH2 domains). Interacts (phosphorylated form) with PLCG1; the interaction is enhanced by hepatocyte growth factor (HGF). Specifically expressed in epithelial cells. Major component of microvilli of intestinal epithelial cells and kidney proximal tubule cells. Expressed in canalicular microvilli of hepatocytes. Belongs to the villin/gelsolin family.
UniProt Protein Details:

Protein type:Actin-binding; Motility/polarity/chemotaxis

Chromosomal Location of Human Ortholog: 2q35

Cellular Component: ruffle; filopodium tip; microvillus; lamellipodium; cytoplasm; actin filament bundle; filopodium

Molecular Function:actin filament binding; identical protein binding; protein binding; phosphatidylinositol-4,5-bisphosphate binding; protein homodimerization activity; caspase inhibitor activity; calcium ion binding

Biological Process: epidermal growth factor receptor signaling pathway; actin filament severing; actin filament depolymerization; actin filament capping; negative regulation of caspase activity; actin nucleation; regulation of cell shape; response to bacterium; epithelial cell differentiation; actin filament polymerization; regulation of actin nucleation; positive regulation of actin filament bundle formation; protein complex assembly; positive regulation of cell migration

NCBI Summary:This gene encodes a member of a family of calcium-regulated actin-binding proteins. This protein represents a dominant part of the brush border cytoskeleton which functions in the capping, severing, and bundling of actin filaments. Two mRNAs of 2.7 kb and 3.5 kb have been observed; they result from utilization of alternate poly-adenylation signals present in the terminal exon. [provided by RefSeq, Jul 2008]
UniProt Code:P09327
NCBI GenInfo Identifier:224471905
NCBI Gene ID:7429
NCBI Accession:P09327.4
UniProt Secondary Accession:P09327,Q53S11, Q96AC8, B2R9A7,
UniProt Related Accession:P09327
Molecular Weight:827
NCBI Full Name:Villin-1
NCBI Synonym Full Names:villin 1
NCBI Official Symbol:VIL1  
NCBI Official Synonym Symbols:VIL; D2S1471  
NCBI Protein Information:villin-1
UniProt Protein Name:Villin-1
Protein Family:VIN3-like protein
UniProt Gene Name:VIL1  
UniProt Entry Name:VILI_HUMAN
Anti-Villin-1 Antibody (CAB4691)Western blot - Villin-1 Rabbit mAb (CAB4691)

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