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Anti-Affinity Gel-conjugated anti DDDDK-Tag Antibody (CABE061)

SKU:
CABE061
Product Type:
Antibody
Host Species:
Mouse
Isotype:
IgG
Antibody Type:
Monoclonal Antibody
€289
Frequently bought together:

Description

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Antibody Name:Anti-Affinity Gel-conjugated anti DDDDK-Tag Antibody
Antibody SKU:CABE061
Antibody Size:100 uL
Application:IP
Reactivity:
Host Species:Mouse
Immunogen:A synthetic peptide corresponding to DDDDK tag.
Application:IP
Recommended Dilution:IP 1:50 - 1:200
Reactivity:
Positive Samples:
Immunogen:A synthetic peptide corresponding to DDDDK tag.
Purification Method:Affinity purification
Storage Buffer:Store at -20°C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:IgG
Sequence:DYKD DDDK
Gene ID:
Uniprot:
Cellular Location:
Calculated MW:
Observed MW:Refer to Figures
Synonyms:DDDDK, DDDDK tag, DDDDK-tag
Background:FLAG-tag, or FLAG octapeptide, or FLAG epitope, is a polypeptide protein tag that can be added to a protein using recombinant DNA technology, having the sequence motif DYKDDDDK. It has been used for studying proteins in living cells and for protein purification by affinity chromatography. It has been used to separate recombinant, overexpressed protein from wild-type protein expressed by the host organism. It can also be used in the isolation of protein complexes with multiple subunits, because its mild purification procedure tends not to disrupt such complexes. It has been used to obtain proteins of sufficient purity and quality to carry out 3D structure determination by x-ray crystallography. A FLAG-tag can be used in many different assays that require recognition by an antibody. If there is no antibody against a given protein, adding a FLAG-tag to a protein allows the protein to be studied with an antibody against the FLAG sequence. Examples are cellular localization studies by immunofluorescence or detection by SDS PAGE protein electrophoresis and Western blotting.

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