Anti-Arylsulfatase F Antibody (CAB8102)

Antibody Name:	ARSF Rabbit Polyclonal Antibody
Antibody SKU:	CAB8102
Antibody Size:	20uL, 50uL, 100uL
Application:	WB IHC
Reactivity:	Human, Mouse, Rat
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 1-200 of human ARSF (NP_004033.2).

Application:	WB IHC
Recommended Dilution:	WB 1:500 - 1:2000 IHC 1:50 - 1:100
Reactivity:	Human, Mouse, Rat
Positive Samples:	293T, A-549, 5637, 22Rv1, BT-474, Mouse liver, Mouse pancreas

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 1-200 of human ARSF (NP_004033.2).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	MRPR RPLV FMSL VCAL LNTC QAHR VHDD KPNI VLIM VDDL GIGD LGCY GNDT MRTP HIDR LARE GVRL TQHI SAAS LCSP SRSA FLTG RYPI RSGM VSSG NRRV IQNL AVPA GLPL NETT LAAL LKKQ GYST GLIG KWHQ GLNC DSRS DQCH HPYN YGFD YYYG MPFT LVDS CWPD PSRN TELA FESQ LWLC VQLV AIAI
Gene ID:	416
Uniprot:	P54793
Cellular Location:	Secreted
Calculated MW:	65kDa
Observed MW:	66kDa

Synonyms:

ARSF, ASF

Background:

This gene is a member of the sulfatase family, and more specifically, the arylsulfatase subfamily. Members of the subfamily share similarity in sequence and splice sites, and are clustered together on chromosome X, suggesting that they are derived from recent gene duplication events. Sulfatases are essential for the correct composition of bone and cartilage matrix. The activity of this protein, unlike that of arylsulfatase E, is not inhibited by warfarin. Multiple alternatively spliced variants, encoding the same protein, have been identified.

UniProt Protein Function:	ARSF: a member of the sulfatase family, and more specifically, the arylsulfatase subfamily. Members of the subfamily share similarity in sequence and splice sites, and are clustered together on chromosome X, suggesting that they are derived from recent gene duplication events. Sulfatases are essential for the correct composition of bone and cartilage matrix. The activity of this protein, unlike that of arylsulfatase E, is not inhibited by warfarin. Multiple alternatively spliced variants, encoding the same protein, have been identified.[provided by RefSeq, Jan 2011]
UniProt Protein Details:	Protein type:Secreted, signal peptide; Secreted; EC 3.1.6.-; Hydrolase Chromosomal Location of Human Ortholog: Xp22.3 Cellular Component: endoplasmic reticulum lumen Molecular Function:arylsulfatase activity Biological Process: glycosphingolipid metabolic process; post-translational protein modification
NCBI Summary:	This gene is a member of the sulfatase family, and more specifically, the arylsulfatase subfamily. Members of the subfamily share similarity in sequence and splice sites, and are clustered together on chromosome X, suggesting that they are derived from recent gene duplication events. Sulfatases are essential for the correct composition of bone and cartilage matrix. The activity of this protein, unlike that of arylsulfatase E, is not inhibited by warfarin. Multiple alternatively spliced variants, encoding the same protein, have been identified.[provided by RefSeq, Jan 2011]
UniProt Code:	P54793
NCBI GenInfo Identifier:	259016386
NCBI Gene ID:	416
NCBI Accession:	P54793.4
UniProt Secondary Accession:	P54793,Q8TCC5,
UniProt Related Accession:	P54793
Molecular Weight:	65,940 Da
NCBI Full Name:	Arylsulfatase F
NCBI Synonym Full Names:	arylsulfatase F
NCBI Official Symbol:	ARSF
NCBI Official Synonym Symbols:	ASF
NCBI Protein Information:	arylsulfatase F
UniProt Protein Name:	Arylsulfatase F
Protein Family:	Arylsulfatase
UniProt Gene Name:	ARSF
UniProt Entry Name:	ARSF_HUMAN

	Western blot analysis of extracts of various cell lines, using ARSF antibody (CAB8102) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 90s.
	Immunohistochemistry of paraffin-embedded rat lung using ARSF antibody (CAB8102) at dilution of 1:100 (40x lens).
	Immunohistochemistry of paraffin-embedded human liver using ARSF antibody (CAB8102) at dilution of 1:100 (40x lens).
	Immunohistochemistry of paraffin-embedded human prostate using ARSF antibody (CAB8102) at dilution of 1:100 (40x lens).
	Immunohistochemistry of paraffin-embedded mouse lung using ARSF antibody (CAB8102) at dilution of 1:100 (40x lens).

Antibodies

Anti-ARSF Antibody (CAB15031)

ARSF Antibody (PACO04302)

Secondary Antibody

Anti-HRP Goat Anti-Rabbit IgG (H+L) Antibody (CABS014)