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Anti-DGKE Antibody (CAB7752)

SKU:
CAB7752
Product Type:
Antibody
Reactivity:
Human
Mouse
Host Species:
Rabbit
Isotype:
IgG
Antibody Type:
Polyclonal Antibody
Research Area:
Cell Biology
  • Cell Biology Antibodies 11 Anti-DGKE Antibody CAB7752
  • Cell Biology Antibodies 11 Anti-DGKE Antibody CAB7752
  • Cell Biology Antibodies 11 Anti-DGKE Antibody CAB7752
  • Cell Biology Antibodies 11 Anti-DGKE Antibody CAB7752
€139 - €419
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Description

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Antibody Name:Anti-DGKE Antibody
Antibody SKU:CAB7752
Antibody Size:20uL, 50uL, 100uL
Application:WB IHC IF
Reactivity:Human, Mouse
Host Species:Rabbit
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 40-260 of human DGKE (NP_003638.1).
Application:WB IHC IF
Recommended Dilution:WB 1:500 - 1:2000 IHC 1:50 - 1:200 IF 1:50 - 1:200
Reactivity:Human, Mouse
Positive Samples:MCF7, HeLa, 293T, Mouse thymus
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 40-260 of human DGKE (NP_003638.1).
Purification Method:Affinity purification
Storage Buffer:Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:IgG
Sequence:CSLQ RSRR QLHR RDIF RKSK HGWR DTDL FSQP TYCC VCAQ HILQ GAFC DCCG LRVD EGCL RKAD KRFQ CKEI MLKN DTKV LDAM PHHW IRGN VPLC SYCM VCKQ QCGC QPKL CDYR CIWC QKTV HDEC MKNS LKNE KCDF GEFK NLII PPSY LTSI NQMR KDKK TDYE VLAS KLGK QWTP LIIL ANSR SGTN MGEG LLGE FRIL LNPV QVFD VTKT PPIK A
Gene ID:8526
Uniprot:P52429
Cellular Location:Cytoplasm, Membrane, Multi-pass membrane protein
Calculated MW:20kDa/63kDa
Observed MW:64kDa
Synonyms:DGKE, AHUS7, DAGK5, DAGK6, DGK, NPHS7
Background:Diacylglycerol kinases are thought to be involved mainly in the regeneration of phosphatidylinositol (PI) from diacylglycerol in the PI-cycle during cell signal transduction. When expressed in mammalian cells, DGK-epsilon shows specificity for arachidonyl-containing diacylglycerol. DGK-epsilon is expressed predominantly in testis.
UniProt Protein Function:DGKE: Highly selective for arachidonate-containing species of diacylglycerol (DAG). May terminate signals transmitted through arachidonoyl-DAG or may contribute to the synthesis of phospholipids with defined fatty acid composition. Belongs to the eukaryotic diacylglycerol kinase family.
UniProt Protein Details:

Protein type:Lipid Metabolism - glycerolipid; Lipid Metabolism - glycerophospholipid; Membrane protein, multi-pass; Membrane protein, integral; EC 2.7.1.107; Motility/polarity/chemotaxis; Kinase, lipid

Chromosomal Location of Human Ortholog: 17q22

Cellular Component: membrane; cytoplasm; integral to membrane; plasma membrane

Molecular Function:metal ion binding; diacylglycerol kinase activity; ATP binding; NAD+ kinase activity

Biological Process: platelet activation; protein kinase C activation; blood coagulation; phosphorylation; phospholipid biosynthetic process

Disease: Nephrotic Syndrome, Type 7

NCBI Summary:Diacylglycerol kinases are thought to be involved mainly in the regeneration of phosphatidylinositol (PI) from diacylglycerol in the PI-cycle during cell signal transduction. When expressed in mammalian cells, DGK-epsilon shows specificity for arachidonyl-containing diacylglycerol. DGK-epsilon is expressed predominantly in testis. [provided by RefSeq, Jul 2008]
UniProt Code:P52429
NCBI GenInfo Identifier:4503313
NCBI Gene ID:8526
NCBI Accession:NP_003638.1
UniProt Related Accession:P52429
Molecular Weight:~ 64kDa
NCBI Full Name:diacylglycerol kinase epsilon
NCBI Synonym Full Names:diacylglycerol kinase epsilon
NCBI Official Symbol:DGKE  
NCBI Official Synonym Symbols:DGK; AHUS7; DAGK5; DAGK6; NPHS7  
NCBI Protein Information:diacylglycerol kinase epsilon
UniProt Protein Name:Diacylglycerol kinase epsilon
UniProt Synonym Protein Names:Diglyceride kinase epsilon
Protein Family:Deoxyguanosine kinase
UniProt Gene Name:DGKE  
UniProt Entry Name:DGKE_HUMAN
Anti-DGKE Antibody (CAB7752)Western blot analysis of extracts of various cell lines, using DGKE antibody (CAB7752) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 30s.
Anti-DGKE Antibody (CAB7752)Immunohistochemistry of paraffin-embedded human gastric using DGKE antibody (CAB7752) at dilution of 1:100 (40x lens).
Anti-DGKE Antibody (CAB7752)Immunohistochemistry of paraffin-embedded mouse kidney using DGKE antibody (CAB7752) at dilution of 1:100 (40x lens).
Anti-DGKE Antibody (CAB7752)Immunofluorescence analysis of U2OS cells using DGKE antibody (CAB7752) at dilution of 1:100. Blue: DAPI for nuclear staining.

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