Anti-FRK Antibody (CAB7556)

Antibody Name:	Anti-FRK Antibody
Antibody SKU:	CAB7556
Antibody Size:	20uL, 50uL, 100uL
Application:	WB IF
Reactivity:	Human, Mouse
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 1-220 of human FRK (NP_002022.1).

Application:	WB IF
Recommended Dilution:	WB 1:500 - 1:2000 IF 1:50 - 1:200
Reactivity:	Human, Mouse
Positive Samples:	HeLa, MCF7, SW620, NCI-H460, HepG2, Mouse kidney

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 1-220 of human FRK (NP_002022.1).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	MSNI CQRL WEYL EPYL PCLS TEAD KSTV IENP GALC SPQS QRHG HYFV ALFD YQAR TAED LSFR AGDK LQVL DTLH EGWW FARH LEKR RDGS SQQL QGYI PSNY VAED RSLQ AEPW FFGA IGRS DAEK QLLY SENK TGSF LIRE SESQ KGEF SLSV LDGA VVKH YRIK RLDE GGFF LTRR RIFS TLNE FVSH YTKT SDGL CVKL GKPC LKIQ VPAP FDLS
Gene ID:	2444
Uniprot:	P42685
Cellular Location:	Cytoplasm, Nucleus
Calculated MW:	41kDa/58kDa
Observed MW:	58kDa

Synonyms:	FRK, GTK, PTK5, RAK
Background:	The protein encoded by this gene belongs to the TYR family of protein kinases. This tyrosine kinase is a nuclear protein and may function during G1 and S phase of the cell cycle and suppress growth.

UniProt Protein Function:	FRK: a tyrosine kinase of the Src family. Expressed in cell lines derived from tissues of lymphoid, brain, breast, colon and bladder origin. A nuclear protein that may function during G1 and S phase of the cell cycle to suppress growth.
UniProt Protein Details:	Protein type:EC 2.7.10.2; Tumor suppressor; Protein kinase, tyrosine (non-receptor); Kinase, protein; Protein kinase, TK; TK group; Src family Chromosomal Location of Human Ortholog: 6q21-q22.3 Cellular Component: cytoplasm; extrinsic to internal side of plasma membrane; intracellular; intracellular membrane-bound organelle; nucleus Molecular Function:protein binding; receptor binding Biological Process: cell differentiation; innate immune response; negative regulation of cell proliferation; negative regulation of transcription from RNA polymerase II promoter; protein amino acid phosphorylation; transmembrane receptor protein tyrosine kinase signaling pathway
NCBI Summary:	The protein encoded by this gene belongs to the TYR family of protein kinases. This tyrosine kinase is a nuclear protein and may function during G1 and S phase of the cell cycle and suppress growth. [provided by RefSeq, Jul 2008]
UniProt Code:	P42685
NCBI GenInfo Identifier:	1169745
NCBI Gene ID:	2444
NCBI Accession:	P42685.1
UniProt Secondary Accession:	P42685,Q13128, Q9NTR5, B4DY49,
UniProt Related Accession:	P42685
Molecular Weight:	41,980 Da
NCBI Full Name:	Tyrosine-protein kinase FRK
NCBI Synonym Full Names:	fyn related Src family tyrosine kinase
NCBI Official Symbol:	FRK
NCBI Official Synonym Symbols:	GTK; RAK; PTK5
NCBI Protein Information:	tyrosine-protein kinase FRK
UniProt Protein Name:	Tyrosine-protein kinase FRK
UniProt Synonym Protein Names:	FYN-related kinase; Nuclear tyrosine protein kinase RAK; Protein-tyrosine kinase 5
Protein Family:	Tyrosine-protein kinase
UniProt Gene Name:	FRK
UniProt Entry Name:	FRK_HUMAN

	Western blot analysis of extracts of various cell lines, using FRK antibody (CAB7556) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 90s.
	Immunofluorescence analysis of A549 cells using FRK antibody (CAB7556). Blue: DAPI for nuclear staining.

Antibodies	ELISA Kits
FRK Antibody (PACO01405)	FRK Colorimetric Cell-Based ELISA
FRK Antibody (PACO09346)

Secondary Antibody

Anti-HRP Goat Anti-Rabbit IgG (H+L) Antibody (CABS014)