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Anti-GPAA1 Antibody (CAB13853)

SKU:
CAB13853
Product Type:
Antibody
Reactivity:
Mouse
Host Species:
Rabbit
Isotype:
IgG
Antibody Type:
Polyclonal Antibody
Research Area:
Metabolism
€139 - €419
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Description

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Antibody Name:Anti-GPAA1 Antibody
Antibody SKU:CAB13853
Antibody Size:20uL, 50uL, 100uL
Application:WB
Reactivity:Mouse
Host Species:Rabbit
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 90-310 of human GPAA1 (NP_003792.1).
Application:WB
Recommended Dilution:WB 1:500 - 1:2000
Reactivity:Mouse
Positive Samples:Mouse heart
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 90-310 of human GPAA1 (NP_003792.1).
Purification Method:Affinity purification
Storage Buffer:Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:IgG
Sequence:AWLE RTMR SVGL EVYT QSFS RKLP FPDE THER YMVS GTNV YGIL RAPR AAST ESLV LTVP CGSD STNS QAVG LLLA LAAH FRGQ IYWA KDIV FLVT EHDL LGTE AWLE AYHD VNVT GMQS SPLQ GRAG AIQA AVAL ELSS DVVT SLDV AVEG LNGQ LPNL DLLN LFQT FCQK GGLL CTLQ GKLQ PEDW TSLD GPLQ GLQT LLLM VLRQ ASGR PHGS HGLF L
Gene ID:8733
Uniprot:O43292
Cellular Location:Endoplasmic reticulum membrane, Multi-pass membrane protein
Calculated MW:61kDa/67kDa
Observed MW:68kDa
Synonyms:GPAA1, GAA1, hGAA1
Background:Posttranslational glycosylphosphatidylinositol (GPI) anchor attachment serves as a general mechanism for linking proteins to the cell surface membrane. The protein encoded by this gene presumably functions in GPI anchoring at the GPI transfer step. The mRNA transcript is ubiquitously expressed in both fetal and adult tissues. The anchor attachment protein 1 contains an N-terminal signal sequence, 1 cAMP- and cGMP-dependent protein kinase phosphorylation site, 1 leucine zipper pattern, 2 potential N-glycosylation sites, and 8 putative transmembrane domains.
UniProt Protein Function:GPAA1: Essential for GPI-anchoring of precursor proteins but not for GPI synthesis. Acts before or during formation of the carbonyl intermediate. 2 isoforms of the human protein are produced by alternative splicing.
UniProt Protein Details:

Protein type:Endoplasmic reticulum; Membrane protein, multi-pass; Glycan Metabolism - glycosylphosphatidylinositol (GPI)-anchor biosynthesis; Membrane protein, integral

Chromosomal Location of Human Ortholog: 8q24.3

Cellular Component: endoplasmic reticulum membrane; GPI-anchor transamidase complex; membrane

Molecular Function:protein binding

Biological Process: attachment of GPI anchor to protein

NCBI Summary:Posttranslational glycosylphosphatidylinositol (GPI) anchor attachment serves as a general mechanism for linking proteins to the cell surface membrane. The protein encoded by this gene presumably functions in GPI anchoring at the GPI transfer step. The mRNA transcript is ubiquitously expressed in both fetal and adult tissues. The anchor attachment protein 1 contains an N-terminal signal sequence, 1 cAMP- and cGMP-dependent protein kinase phosphorylation site, 1 leucine zipper pattern, 2 potential N-glycosylation sites, and 8 putative transmembrane domains. [provided by RefSeq, Jul 2008]
UniProt Code:O43292
NCBI GenInfo Identifier:44887920
NCBI Gene ID:8733
NCBI Accession:O43292.3
UniProt Secondary Accession:O43292,Q9NSS0, Q9UQ31,
UniProt Related Accession:O43292
Molecular Weight:61,098 Da
NCBI Full Name:Glycosylphosphatidylinositol anchor attachment 1 protein
NCBI Synonym Full Names:glycosylphosphatidylinositol anchor attachment 1
NCBI Official Symbol:GPAA1  
NCBI Official Synonym Symbols:GAA1; hGAA1  
NCBI Protein Information:glycosylphosphatidylinositol anchor attachment 1 protein
UniProt Protein Name:Glycosylphosphatidylinositol anchor attachment 1 protein
UniProt Synonym Protein Names:GAA1 protein homolog; hGAA1
Protein Family:Glycosylphosphatidylinositol anchor attachment 1 protein
UniProt Gene Name:GPAA1  
UniProt Entry Name:GPAA1_HUMAN
Anti-GPAA1 Antibody (CAB13853)Western blot analysis of extracts of mouse heart, using GPAA1 antibody (CAB13853) at 1:3000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 1s.

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