Anti-HSPA13 Antibody (CAB4132)

Antibody Name:	Anti-HSPA13 Antibody
Antibody SKU:	CAB4132
Antibody Size:	20uL, 50uL, 100uL
Application:	WB IF
Reactivity:	Human, Mouse, Rat
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 165-471 of human HSPA13 (NP_008879.3).

Application:	WB IF
Recommended Dilution:	WB 1:500 - 1:2000 IF 1:50 - 1:100
Reactivity:	Human, Mouse, Rat
Positive Samples:	U-87MG

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 165-471 of human HSPA13 (NP_008879.3).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	MPVA NAVI SVPA EFDL KQRN STIE AANL AGLK ILRV INEP TAAA MAYG LHKA DVFH VLVI DLGG GTLD VSLL NKQG GMFL TRAM SGNN KLGG QDFN QRLL QYLY KQIY QTYG FVPS RKEE IHRL RQAV EMVK LNLT LHQS AQLS VLLT VEEQ DRKE PHSS DTEL PKDK LSSA DDHR VNSG FGRG LSDK KSGE SQVL FETE ISRK LFDT LNED LFQK ILVP IQQV LKEG HLEK TEID EVVL VGGS TRIP RIRQ VIQE FFGK DPNT SVDP DLAV VTGV AIQA GIDG GSWP LQVS ALEI PNKH LQKT NFN
Gene ID:	6782
Uniprot:	P48723
Cellular Location:	Endoplasmic reticulum, Microsome
Calculated MW:	51kDa
Observed MW:	65kDa

Synonyms:	HSPA13, STCH
Background:	The protein encoded by this gene is a member of the heat shock protein 70 family and is found associated with microsomes. Members of this protein family play a role in the processing of cytosolic and secretory proteins, as well as in the removal of denatured or incorrectly-folded proteins. The encoded protein contains an ATPase domain and has been shown to associate with a ubiquitin-like protein.

UniProt Protein Function:	HSPA13: Has peptide-independent ATPase activity. Belongs to the heat shock protein 70 family.
UniProt Protein Details:	Protein type:Heat shock protein Chromosomal Location of Human Ortholog: 21q11 Cellular Component: intracellular membrane-bound organelle; endoplasmic reticulum Molecular Function:ATP binding
NCBI Summary:	The protein encoded by this gene is a member of the heat shock protein 70 family and is found associated with microsomes. Members of this protein family play a role in the processing of cytosolic and secretory proteins, as well as in the removal of denatured or incorrectly-folded proteins. The encoded protein contains an ATPase domain and has been shown to associate with a ubiquitin-like protein. [provided by RefSeq, Jul 2008]
UniProt Code:	P48723
NCBI GenInfo Identifier:	1351125
NCBI Gene ID:	6782
NCBI Accession:	P48723.1
UniProt Secondary Accession:	P48723,Q8NE40, B2R616,
UniProt Related Accession:	P48723
Molecular Weight:	51,927 Da
NCBI Full Name:	Heat shock 70 kDa protein 13
NCBI Synonym Full Names:	heat shock protein 70kDa family, member 13
NCBI Official Symbol:	HSPA13
NCBI Official Synonym Symbols:	STCH
NCBI Protein Information:	heat shock 70 kDa protein 13; microsomal stress 70 protein ATPase core; microsomal stress-70 protein ATPase core; stress 70 protein chaperone, microsome-associated, 60kD; stress 70 protein chaperone, microsome-associated, 60kDa; stress-70 protein chaperone microsome-associated 60 kDa protein
UniProt Protein Name:	Heat shock 70 kDa protein 13
UniProt Synonym Protein Names:	Microsomal stress-70 protein ATPase core; Stress-70 protein chaperone microsome-associated 60 kDa protein
Protein Family:	Heat shock 70 kDa protein
UniProt Gene Name:	HSPA13
UniProt Entry Name:	HSP13_HUMAN

	Western blot analysis of extracts of U-87MG cells, using HSPA13 antibody (CAB4132) at 1:3000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit (RM00021). Exposure time: 90s.
	Immunofluorescence analysis of C6 cells using HSPA13 Polyclonal Antibody (CAB4132) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
	Immunofluorescence analysis of L929 cells using HSPA13 Polyclonal Antibody (CAB4132) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

Antibodies

HSPA13 Antibody (PACO09823)

Secondary Antibody

Anti-HRP Goat Anti-Rabbit IgG (H+L) Antibody (CABS014)