Anti-INSL3 Antibody (CAB5728)

Antibody Name:	Anti-INSL3 Antibody
Antibody SKU:	CAB5728
Antibody Size:	20uL, 50uL, 100uL
Application:	WB IHC IF
Reactivity:	Human, Mouse, Rat
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 1-131 of human INSL3 (NP_005534.2).

Application:	WB IHC IF
Recommended Dilution:	WB 1:500 - 1:2000 IHC 1:50 - 1:100 IF 1:50 - 1:100
Reactivity:	Human, Mouse, Rat
Positive Samples:	U-937

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 1-131 of human INSL3 (NP_005534.2).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	MDPR LPAW ALVL LGPA LVFA LGPA PTPE MREK LCGH HFVR ALVR VCGG PRWS TEAR RPAT GGDR ELLQ WLER RHLL HGLV ADSN LTLG PGLQ PLPQ TSHH HRHH RAAA TNPA RYCC LSGC TQQD LLTL CPY
Gene ID:	3640
Uniprot:	P51460
Cellular Location:	Secreted
Calculated MW:	14kDa/16kDa
Observed MW:	15kDa

Synonyms:

INSL3, RLF, RLNL, ley-I-L

Background:

This gene encodes a member of the insulin-like hormone superfamily. The encoded protein is mainly produced in gonadal tissues. Studies of the mouse counterpart suggest that this gene may be involved in the development of urogenital tract and female fertility. This protein may also act as a hormone to regulate growth and differentiation of gubernaculum, and thus mediating intra-abdominal testicular descent. Mutations in this gene may lead to cryptorchidism. Alternate splicing results in multiple transcript variants.

UniProt Protein Function:	INSL3: Seems to play a role in testicular function. May be a trophic hormone with a role in testicular descent in fetal life. Is a ligand for LGR8 receptor. Defects in INSL3 seems to be a cause of cryptorchidism (CRYPTO); also known as impaired testicular descent. It is one of the most frequent congenital abnormalities in humans, involving 2-5% of male births. Cryptorchidism is associated with increased risk of infertility and testicular cancer. The frequency of INSL3 gene mutations as a cause of cryptorchidism is low. Belongs to the insulin family.
UniProt Protein Details:	Protein type:Secreted; Secreted, signal peptide Chromosomal Location of Human Ortholog: 19p13.2-p12 Cellular Component: extracellular space; perinuclear region of cytoplasm; extracellular region Molecular Function:G-protein-coupled receptor binding; protease binding; hormone activity; insulin receptor binding; receptor binding Biological Process: negative regulation of cell proliferation; cell-cell signaling; in utero embryonic development; positive regulation of cAMP biosynthetic process; positive regulation of cell proliferation; male gonad development; spermatogenesis; G-protein signaling, adenylate cyclase inhibiting pathway; oocyte maturation; negative regulation of apoptosis Disease: Cryptorchidism, Unilateral Or Bilateral
NCBI Summary:	This gene encodes a member of the insulin-like hormone superfamily. The encoded protein is mainly produced in gonadal tissues. Studies of the mouse counterpart suggest that this gene may be involved in the development of urogenital tract and female fertility. This protein may also act as a hormone to regulate growth and differentiation of gubernaculum, and thus mediating intra-abdominal testicular descent. Mutations in this gene may lead to cryptorchidism. Alternate splicing results in multiple transcript variants. [provided by RefSeq, May 2012]
UniProt Code:	P51460
NCBI GenInfo Identifier:	317373369
NCBI Gene ID:	3640
NCBI Accession:	P51460.2
UniProt Secondary Accession:	P51460,Q3KPI5, Q3KPI6, Q6YNB5, Q9UEA2, Q9UPH6, B4DZ72 G3XAG0,
UniProt Related Accession:	P51460
Molecular Weight:	16,872 Da
NCBI Full Name:	Insulin-like 3
NCBI Synonym Full Names:	insulin-like 3 (Leydig cell)
NCBI Official Symbol:	INSL3
NCBI Official Synonym Symbols:	RLF; RLNL; ley-I-L
NCBI Protein Information:	insulin-like 3; prepro-INSL3; relaxin-like factor b; leydig insulin-like peptide; leydig insulin -like hormone
UniProt Protein Name:	Insulin-like 3
UniProt Synonym Protein Names:	Leydig insulin-like peptide; Ley-I-L; Relaxin-like factorCleaved into the following 2 chains:Insulin-like 3 B chain; Insulin-like 3 A chain
Protein Family:	Insulin
UniProt Gene Name:	INSL3
UniProt Entry Name:	INSL3_HUMAN

	Western blot analysis of extracts of U-937 cells, using INSL3 antibody (CAB5728) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST.
	Immunohistochemistry of paraffin-embedded human prostate using INSL3 antibody (CAB5728) at dilution of 1:100 (40x lens).

Antibodies	ELISA Kits
INSL3 Antibody (PACO07345)	Human INSL3 / Insulin-like 3 ELISA Kit
	Human INSL3 (Insulin Like Protein 3) CLIA Kit (HUES00290)
	Human INSL3(Insulin Like Protein 3)ELISA Kit (HUES01620)
	Human Insulin-like 3 (INSL3) ELISA Kit

Secondary Antibody

Anti-HRP Goat Anti-Rabbit IgG (H+L) Antibody (CABS014)