Anti-KCNJ1 Antibody (CAB8554)

Antibody Name:	Anti-KCNJ1 Antibody
Antibody SKU:	CAB8554
Antibody Size:	20uL, 50uL, 100uL
Application:	WB IHC
Reactivity:	Mouse, Rat
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 182-391 of human KCNJ1 (NP_000211.1).

Application:	WB IHC
Recommended Dilution:	WB 1:500 - 1:2000 IHC 1:50 - 1:200
Reactivity:	Mouse, Rat
Positive Samples:	Mouse kidney

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 182-391 of human KCNJ1 (NP_000211.1).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	ISRP KKRA KTIT FSKN AVIS KRGG KLCL LIRV ANLR KSLL IGSH IYGK LLKT TVTP EGET IILD QINI NFVV DAGN ENLF FISP LTIY HVID HNSP FFHM AAET LLQQ DFEL VVFL DGTV ESTS ATCQ VRTS YVPE EVLW GYRF APIV SKTK EGKY RVDF HNFS KTVE VETP HCAM CLYN EKDV RARM KRGY DNPN FILS EVNE TDDT KM
Gene ID:	3758
Uniprot:	P48048
Cellular Location:	Cell membrane, Multi-pass membrane protein
Calculated MW:	42kDa/44kDa
Observed MW:	45kDa

Synonyms:

KCNJ1, KIR1.1, ROMK, ROMK1

Background:

Potassium channels are present in most mammalian cells, where they participate in a wide range of physiologic responses. The protein encoded by this gene is an integral membrane protein and inward-rectifier type potassium channel. It is activated by internal ATP and probably plays an important role in potassium homeostasis. The encoded protein has a greater tendency to allow potassium to flow into a cell rather than out of a cell. Mutations in this gene have been associated with antenatal Bartter syndrome, which is characterized by salt wasting, hypokalemic alkalosis, hypercalciuria, and low blood pressure. Multiple transcript variants encoding different isoforms have been found for this gene.

UniProt Protein Function:	In the kidney, probably plays a major role in potassium homeostasis. Inward rectifier potassium channels are characterized by a greater tendency to allow potassium to flow into the cell rather than out of it. Their voltage dependence is regulated by the concentration of extracellular potassium; as external potassium is raised, the voltage range of the channel opening shifts to more positive voltages. The inward rectification is mainly due to the blockage of outward current by internal magnesium. This channel is activated by internal ATP and can be blocked by external barium.
NCBI Summary:	Potassium channels are present in most mammalian cells, where they participate in a wide range of physiologic responses. The protein encoded by this gene is an integral membrane protein and inward-rectifier type potassium channel. It is activated by internal ATP and probably plays an important role in potassium homeostasis. The encoded protein has a greater tendency to allow potassium to flow into a cell rather than out of a cell. Mutations in this gene have been associated with antenatal Bartter syndrome, which is characterized by salt wasting, hypokalemic alkalosis, hypercalciuria, and low blood pressure. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008]
UniProt Code:	P48048
NCBI GenInfo Identifier:	1352479
NCBI Gene ID:	3758
NCBI Accession:	P48048.1
UniProt Secondary Accession:	P48048,Q6LD67, B2RMR4,
UniProt Related Accession:	P48048
Molecular Weight:	44,611 Da
NCBI Full Name:	ATP-sensitive inward rectifier potassium channel 1
NCBI Synonym Full Names:	potassium voltage-gated channel subfamily J member 1
NCBI Official Symbol:	KCNJ1
NCBI Official Synonym Symbols:	ROMK; ROMK1; KIR1.1
NCBI Protein Information:	ATP-sensitive inward rectifier potassium channel 1
UniProt Protein Name:	ATP-sensitive inward rectifier potassium channel 1
UniProt Synonym Protein Names:	ATP-regulated potassium channel ROM-K; Inward rectifier K(+) channel Kir1.1; Potassium channel, inwardly rectifying subfamily J member 1
Protein Family:	ATP-sensitive inward rectifier potassium channel
UniProt Gene Name:	KCNJ1
UniProt Entry Name:	KCNJ1_HUMAN

	Western blot analysis of extracts of mouse kidney, using KCNJ1 antibody (CAB8554) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 30s.
	Immunohistochemistry of paraffin-embedded rat testis using KCNJ1 antibody (CAB8554) at dilution of 1:100 (40x lens).
	Immunohistochemistry of paraffin-embedded rat pancreas using KCNJ1 antibody (CAB8554) at dilution of 1:100 (40x lens).

Antibodies

Phospho-KCNJ1 (S44) Antibody (PACO05969)

KCNJ1 Antibody (PACO05970)

KCNJ1 Antibody (PACO10056)

Secondary Antibody

Anti-HRP Goat Anti-Rabbit IgG (H+L) Antibody (CABS014)