Anti-KCNK10 Antibody (CAB11681)

Antibody Name:	Anti-KCNK10 Antibody
Antibody SKU:	CAB11681
Antibody Size:	20uL, 50uL, 100uL
Application:	WB
Reactivity:	Mouse, Rat
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 384-543 of human KCNK10 (NP_612191.1).

Application:	WB
Recommended Dilution:	WB 1:500 - 1:2000
Reactivity:	Mouse, Rat
Positive Samples:	Mouse spleen, Rat testis

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 384-543 of human KCNK10 (NP_612191.1).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	RLGL DQRA HSLD MLSP EKRS VFAA LDTG RFKA SSQE SINN RPNN LRLK GPEQ LNKH GQGA SEDN IINK FGST SRLT KRKN KDLK KTLP EDVQ KIYK TFRN YSLD EEKK EEET EKMC NSDN SSTA MLTD CIQQ HAEL ENGM IPTD TKDR EPEN NSLL EDRN
Gene ID:	54207
Uniprot:	P57789
Cellular Location:	Membrane, Multi-pass membrane protein
Calculated MW:	59kDa/60kDa
Observed MW:	65kDa

Synonyms:

KCNK10, K2p10.1, PPP1R97, TREK-2, TREK2

Background:

The protein encoded by this gene belongs to the family of potassium channel proteins containing two pore-forming P domains. This channel is an open rectifier which primarily passes outward current under physiological K+ concentrations, and is stimulated strongly by arachidonic acid and to a lesser degree by membrane stretching, intracellular acidification, and general anaesthetics. Several alternatively spliced transcript variants encoding different isoforms have been identified for this gene.

UniProt Protein Function:	TREK-2: Outward rectifying potassium channel. Produces rapidly activating and non-inactivating outward rectifier K(+) currents. Activated by arachidonic acid and other naturally occurring unsaturated free fatty acids. Belongs to the two pore domain potassium channel (TC 1.A.1.8) family. 3 isoforms of the human protein are produced by alternative splicing.
UniProt Protein Details:	Protein type:Channel, potassium; Membrane protein, multi-pass; Membrane protein, integral Chromosomal Location of Human Ortholog: 14q31.3 Cellular Component: integral to plasma membrane; plasma membrane Molecular Function:potassium channel activity; potassium ion leak channel activity; voltage-gated ion channel activity Biological Process: memory; signal transduction; stabilization of membrane potential; synaptic transmission; transport
NCBI Summary:	The protein encoded by this gene belongs to the family of potassium channel proteins containing two pore-forming P domains. This channel is an open rectifier which primarily passes outward current under physiological K+ concentrations, and is stimulated strongly by arachidonic acid and to a lesser degree by membrane stretching, intracellular acidification, and general anaesthetics. Several alternatively spliced transcript variants encoding different isoforms have been identified for this gene. [provided by RefSeq, Sep 2008]
UniProt Code:	P57789
NCBI GenInfo Identifier:	13431412
NCBI Gene ID:	54207
NCBI Accession:	P57789.1
UniProt Secondary Accession:	P57789,Q6B014, Q8TDK7, Q8TDK8, Q9HB59, B2R8T4, B2RCT3 B5TJL4,
UniProt Related Accession:	P57789
Molecular Weight:
NCBI Full Name:	Potassium channel subfamily K member 10
NCBI Synonym Full Names:	potassium two pore domain channel subfamily K member 10
NCBI Official Symbol:	KCNK10
NCBI Official Synonym Symbols:	TREK2; TREK-2; K2p10.1; PPP1R97
NCBI Protein Information:	potassium channel subfamily K member 10
UniProt Protein Name:	Potassium channel subfamily K member 10
UniProt Synonym Protein Names:	Outward rectifying potassium channel protein TREK-2; TREK-2 K(+) channel subunit
Protein Family:	Potassium channel subfamily
UniProt Gene Name:	KCNK10
UniProt Entry Name:	KCNKA_HUMAN

Western blot analysis of extracts of various cell lines, using KCNK10 antibody (CAB11681) at 1:3000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 60s.

Antibodies

Anti-KCNK10 Antibody (CAB11696)

KCNK10 Antibody (PACO07181)

Secondary Antibody

Anti-HRP Goat Anti-Rabbit IgG (H+L) Antibody (CABS014)