Anti-OTUD6B Antibody (CAB14511)

Antibody Name:	Anti-OTUD6B Antibody
Antibody SKU:	CAB14511
Antibody Size:	20uL, 50uL, 100uL
Application:	WB
Reactivity:	Human, Mouse, Rat
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 31-323 of human OTUD6B (NP_057107.3).

Application:	WB
Recommended Dilution:	WB 1:500 - 1:2000
Reactivity:	Human, Mouse, Rat
Positive Samples:	U-87MG, Raji, 293T, A-431, Jurkat, Mouse brain, Mouse heart, Rat liver

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 31-323 of human OTUD6B (NP_057107.3).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	MEAV LTEE LDEE EQLL RRHR KEKK ELQA KIQG MKNA VPKN DKKR RKQL TEDV AKLE KEME QKHR EELE QLKL TTKE NKID SVAV NISN LVLE NQPP RISK AQKR REKK AALE KERE ERIA EAEI ENLT GARH MESE KLAQ ILAA RQLE IKQI PSDG HCMY KAIE DQLK EKDC ALTV VALR SQTA EYMQ SHVE DFLP FLTN PNTG DMYT PEEF QKYC EDIV NTAA WGGQ LELR ALSH ILQT PIEI IQAD SPPI IVGE EYSK KPLI LVYM RHAY GLGE HYNS VTRL VNIV TENC S
Gene ID:	51633
Uniprot:	Q8N6M0
Cellular Location:
Calculated MW:	21kDa/33kDa
Observed MW:	38kDa

Synonyms:	OTUD6B, CGI-77, DUBA-5, DUBA5, IDDFSDA
Background:	This gene encodes a member of the ovarian tumor domain (OTU)-containing subfamily of deubiquitinating enzymes. Deubiquitinating enzymes are primarily involved in removing ubiquitin from proteins targeted for degradation. This protein may function as a negative regulator of the cell cycle in B cells.

UniProt Protein Function:	OTUD6B: 2 isoforms of the human protein are produced by alternative splicing
UniProt Protein Details:	Protein type:Ubiquitin-specific protease; EC 3.4.19.12 Chromosomal Location of Human Ortholog: 8q21.3
NCBI Summary:	This gene encodes a member of the ovarian tumor domain (OTU)-containing subfamily of deubiquitinating enzymes. Deubiquitinating enzymes are primarily involved in removing ubiquitin from proteins targeted for degradation. This protein may function as a negative regulator of the cell cycle in B cells. [provided by RefSeq, Nov 2013]
UniProt Code:	Q8N6M0
NCBI GenInfo Identifier:	20987238
NCBI Gene ID:	51633
NCBI Accession:	BC029760
UniProt Secondary Accession:	Q8N6M0,Q9NTA4, Q9Y387, A8K6I1, B4DEY0,
UniProt Related Accession:	Q8N6M0
Molecular Weight:	21,809 Da
NCBI Full Name:	Homo sapiens OTU domain containing 6B, mRNA
NCBI Synonym Full Names:	OTU domain containing 6B
NCBI Official Symbol:	OTUD6B
NCBI Official Synonym Symbols:	DUBA5; CGI-77; DUBA-5
NCBI Protein Information:	OTU domain-containing protein 6B
UniProt Protein Name:	OTU domain-containing protein 6B
UniProt Synonym Protein Names:	DUBA-5
Protein Family:	OTU domain-containing protein
UniProt Gene Name:	OTUD6B
UniProt Entry Name:	OTU6B_HUMAN

	Western blot analysis of extracts of various cell lines, using OTUD6B antibody (CAB14511) at 1:3000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 30s.
	Immunoprecipitation analysis of 200ug extracts of U-87MG cells using 3ug OTUD6B antibody (CAB14511). Western blot was performed from the immunoprecipitate using OTUD6B antibody (CAB14511) at a dilition of 1:1000.

Secondary Antibody