Anti-PIWIL-3 Antibody (CAB13203)

Antibody Name:	PIWIL3 Rabbit Polyclonal Antibody
Antibody SKU:	CAB13203
Antibody Size:	20uL, 50uL, 100uL
Application:	WB
Reactivity:	Mouse, Rat
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 40-320 of human PIWIL3 (NP_001242904.1).

Application:	WB
Recommended Dilution:	WB 1:500 - 1:2000
Reactivity:	Mouse, Rat
Positive Samples:	Mouse testis, Rat testis

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 40-320 of human PIWIL3 (NP_001242904.1).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	QLQS TPRP LQEE VPVV RPLQ PRAA RGGA GGGA QSQG VKEP GPEA GLHT APLQ ERRI GGVF QDLV VNTR QDMK HVKD SKTG SEGT VVQL LANH FRVI SRPQ WVAY KYNV DYKP DIED GNLR TILL DQHR RKFG ERHI FDGN SLLL SRPL KERR VEWL STTK DKNI VKIT VEFS KELT PTSP DCLR YYNI LFRR TFKL LDFE QVGR NYYT KKKA IQLY RHGT SLEI WLGY VTSV LQYE NSIT LCAD VSHK LLRI ETAY DFIK RTSA QAQT GNIR EEVT NKLI G
Gene ID:	440822
Uniprot:	Q7Z3Z3
Cellular Location:	Cytoplasm
Calculated MW:	101kDa
Observed MW:	101kDa

Synonyms:	PIWIL3, HIWI3
Background:	This gene encodes a member of the PIWI subfamily of Argonaute family proteins. This subfamily of proteins contains a PAZ domain, found in proteins involved in RNA-mediated gene silencing, and a C-terminal Piwi domain. The encoded protein is thought to function in maintenance of germline cells. Multiple transcript variants encoding different isoforms have been found for this gene.

UniProt Protein Function:	HTRA3: Serine protease that cleaves beta-casein/CSN2 as well as several extracellular matrix (ECM) proteoglycans such as decorin/DCN, biglycan/BGN and fibronectin/FN1. Inhibits signaling mediated by TGF-beta family proteins possibly indirectly by degradation of these ECM proteoglycans. May act as a tumor suppressor. Negatively regulates, in vitro, trophoblast invasion during placental development and may be involved in the development of the placenta in vivo. May also have a role in ovarian development, granulosa cell differentiation and luteinization. Belongs to the peptidase S1B family. 2 isoforms of the human protein are produced by alternative splicing.
UniProt Protein Details:	Protein type:EC 3.4.21.-; Secreted, signal peptide; Protease; Secreted Chromosomal Location of Human Ortholog: 4p16.1 Cellular Component: extracellular region Molecular Function:insulin-like growth factor binding; protein binding; serine-type peptidase activity; serine-type endopeptidase activity; endopeptidase activity Biological Process: regulation of cell growth; negative regulation of transforming growth factor beta receptor signaling pathway; proteolysis; negative regulation of BMP signaling pathway
NCBI Summary:	This gene encodes a member of the PIWI subfamily of Argonaute family proteins. This subfamily of proteins contains a PAZ domain, found in proteins involved in RNA-mediated gene silencing, and a C-terminal Piwi domain. The encoded protein is thought to function in maintenance of germline cells. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Dec 2011]
UniProt Code:	Q7Z3Z3
NCBI GenInfo Identifier:	153792298
NCBI Gene ID:	440822
NCBI Accession:	NP_001008496
UniProt Related Accession:	Q7Z3Z3
Molecular Weight:	101,089 Da
NCBI Full Name:	piwi-like protein 3 isoform 1
NCBI Synonym Full Names:	piwi-like RNA-mediated gene silencing 3
NCBI Official Symbol:	PIWIL3
NCBI Official Synonym Symbols:	HIWI3
NCBI Protein Information:	piwi-like protein 3; piwi-like 3
UniProt Protein Name:	Serine protease HTRA3
UniProt Synonym Protein Names:	High-temperature requirement factor A3; Pregnancy-related serine protease
Protein Family:	Serine protease
UniProt Gene Name:	HTRA3
UniProt Entry Name:	HTRA3_HUMAN

Western blot analysis of extracts of various cell lines, using PIWIL3 antibody (CAB13203) at 1:3000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 90s.

Secondary Antibody

Anti-HRP Goat Anti-Rabbit IgG (H+L) Antibody (CABS014)