Anti-PSMD5 Antibody (CAB6954)

Antibody Name:	Anti-PSMD5 Antibody
Antibody SKU:	CAB6954
Antibody Size:	20uL, 50uL, 100uL
Application:	WB IHC
Reactivity:	Human, Mouse, Rat
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 255-504 of human PSMD5 (NP_005038.1).

Application:	WB IHC
Recommended Dilution:	WB 1:500 - 1:2000 IHC 1:50 - 1:200
Reactivity:	Human, Mouse, Rat
Positive Samples:	A-431, BT-474, HeLa, Mouse liver, Rat liver

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 255-504 of human PSMD5 (NP_005038.1).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	NIIV GADS DPFS SFYL PGFV KFFG NLAV MDSP QQIC ERYP IFVE KVFE MIES QDPT MIGV AVDT VGIL GSNV EGKQ VLQK TGTR FERL LMRI GHQS KNAP VELK IRCL DAIS SLLY LPPE QQTD DLLR MTES WFSS LSRD PLEL FRGI SSQP FPEL HCAA LKVF TAIA NQPW AQKL MFNS PGFV EYVV DRSV EHDK ASKD AKYE LVKA LANS KTIA EIFG NPNY LRLR TYLS EGPY YVKP VSTT AVEG AE
Gene ID:	5711
Uniprot:	Q16401
Cellular Location:
Calculated MW:	51kDa/56kDa
Observed MW:	56kDa

Synonyms:

PSMD5, S5B

Background:

The 26S proteasome is a multicatalytic proteinase complex with a highly ordered structure composed of 2 complexes, a 20S core and a 19S regulator. The 20S core is composed of 4 rings of 28 non-identical subunits; 2 rings are composed of 7 alpha subunits and 2 rings are composed of 7 beta subunits. The 19S regulator is composed of a base, which contains 6 ATPase subunits and 2 non-ATPase subunits, and a lid, which contains up to 10 non-ATPase subunits. Proteasomes are distributed throughout eukaryotic cells at a high concentration and cleave peptides in an ATP/ubiquitin-dependent process in a non-lysosomal pathway. This gene encodes a non-ATPase subunit of the 19S regulator base that functions as a chaperone protein during 26S proteasome assembly.

UniProt Protein Function:	PSMD5: Acts as a chaperone during the assembly of the 26S proteasome, specifically of the base subcomplex of the PA700/19S regulatory complex (RC). In the initial step of the base subcomplex assembly is part of an intermediate PSMD5:PSMC2:PSMC1:PSMD2 module which probably assembles with a PSMD10:PSMC4:PSMC5:PAAF1 module followed by dissociation of PSMD5. Belongs to the proteasome subunit S5B/HSM3 family.
UniProt Protein Details:	Protein type:Protease; Proteasome complex Chromosomal Location of Human Ortholog: 9q33.2 Cellular Component: nucleoplasm; proteasome complex; cytosol Molecular Function:protein binding Biological Process: positive regulation of ubiquitin-protein ligase activity during mitotic cell cycle; negative regulation of ubiquitin-protein ligase activity during mitotic cell cycle; protein polyubiquitination; viral reproduction; apoptosis; antigen processing and presentation of exogenous peptide antigen via MHC class I, TAP-dependent; DNA damage response, signal transduction by p53 class mediator resulting in cell cycle arrest; regulation of apoptosis; antigen processing and presentation of peptide antigen via MHC class I; regulation of ubiquitin-protein ligase activity during mitotic cell cycle; anaphase-promoting complex-dependent proteasomal ubiquitin-dependent protein catabolic process; antigen processing and presentation of exogenous peptide antigen via MHC class I; gene expression; mitotic cell cycle; regulation of amino acid metabolic process; G1/S transition of mitotic cell cycle; negative regulation of apoptosis
NCBI Summary:	The 26S proteasome is a multicatalytic proteinase complex with a highly ordered structure composed of 2 complexes, a 20S core and a 19S regulator. The 20S core is composed of 4 rings of 28 non-identical subunits; 2 rings are composed of 7 alpha subunits and 2 rings are composed of 7 beta subunits. The 19S regulator is composed of a base, which contains 6 ATPase subunits and 2 non-ATPase subunits, and a lid, which contains up to 10 non-ATPase subunits. Proteasomes are distributed throughout eukaryotic cells at a high concentration and cleave peptides in an ATP/ubiquitin-dependent process in a non-lysosomal pathway. This gene encodes a non-ATPase subunit of the 19S regulator base that functions as a chaperone protein during 26S proteasome assembly. [provided by RefSeq, Jul 2012]
UniProt Code:	Q16401
NCBI GenInfo Identifier:	3122657
NCBI Gene ID:	5711
NCBI Accession:	Q16401.3
UniProt Related Accession:	Q16401
Molecular Weight:
NCBI Full Name:	26S proteasome non-ATPase regulatory subunit 5
NCBI Synonym Full Names:	proteasome 26S subunit, non-ATPase 5
NCBI Official Symbol:	PSMD5
NCBI Official Synonym Symbols:	S5B
NCBI Protein Information:	26S proteasome non-ATPase regulatory subunit 5
UniProt Protein Name:	26S proteasome non-ATPase regulatory subunit 5
UniProt Synonym Protein Names:	26S protease subunit S5 basic; 26S proteasome subunit S5B
Protein Family:	26S proteasome non-ATPase regulatory
UniProt Gene Name:	PSMD5
UniProt Entry Name:	PSMD5_HUMAN

	Western blot analysis of extracts of various cell lines, using PSMD5 antibody (CAB6954) at 1:2000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 90s.
	Immunohistochemistry of paraffin-embedded rat kidney using PSMD5 antibody (CAB6954) at dilution of 1:100 (40x lens).

Antibodies

PSMD5 Antibody (PACO11553)

Secondary Antibody

Anti-HRP Goat Anti-Rabbit IgG (H+L) Antibody (CABS014)