Anti-Rabbit anti DDDDK-Tag Polyclonal Antibody (CABE004)
- SKU:
- CABE004
- Product Type:
- Antibody
- Host Species:
- Rabbit
- Isotype:
- IgG
- Antibody Type:
- Polyclonal Antibody
Frequently bought together:
Description
| Antibody Name: | Anti-Rabbit anti DDDDK-Tag Polyclonal Antibody |
| Antibody SKU: | CABE004 |
| Antibody Size: | 100 uL |
| Application: | WB IHC IF IP |
| Reactivity: | |
| Host Species: | Rabbit |
| Immunogen: | A synthetic peptide corresponding to DDDDK tag. |
| Application: | WB IHC IF IP |
| Recommended Dilution: | WB 1:1000 - 1:2000 IHC 1:50 - 1:100 IF 1:50 - 1:100 IP 1:50 - 1:100 |
| Reactivity: | |
| Positive Samples: | 293T |
| Immunogen: | A synthetic peptide corresponding to DDDDK tag. |
| Purification Method: | Affinity purification |
| Storage Buffer: | Store at -20°C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Isotype: | IgG |
| Sequence: | DYKD DDDK |
| Gene ID: | |
| Uniprot: | |
| Cellular Location: | |
| Calculated MW: | |
| Observed MW: | 50KDa |
 | Western blot analysis of 293T-pCMV3-GFP-Flag-Myc-HA-His protein using Rabbit anti DDDDK-Tag pAb (CABE004) at different dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. |
 | Immunofluorescence analysis of HeLa cells transfected with DDDDK-Tag (top left) and untreated HeLa cells (top right) using Rabbit anti DDDDK-Tag(red, AE004) at dilution of 1:100. Blue: DAPI for nuclear staining. |
 | Immunoprecipitation analysis of 200ug extracts of 293T cells using 3ug Rabbit anti DDDDK-Tag antibody (CABE004). Western blot was performed from the immunoprecipitate using Rabbit anti DDDDK-Tag antibody (CABE004) at a dilition of 1:1000. |
| Synonyms: | DDDDK, DDDDK tag, DDDDK-tag |
| Background: | FLAG-tag, or FLAG octapeptide, or FLAG epitope, is a polypeptide protein tag that can be added to a protein using recombinant DNA technology, having the sequence motif DYKDDDDK. It has been used for studying proteins in living cells and for protein purification by affinity chromatography. It has been used to separate recombinant, overexpressed protein from wild-type protein expressed by the host organism. It can also be used in the isolation of protein complexes with multiple subunits, because its mild purification procedure tends not to disrupt such complexes. It has been used to obtain proteins of sufficient purity and quality to carry out 3D structure determination by x-ray crystallography.A FLAG-tag can be used in many different assays that require recognition by an antibody. If there is no antibody against a given protein, adding a FLAG-tag to a protein allows the protein to be studied with an antibody against the FLAG sequence. Examples are cellular localization studies by immunofluorescence or detection by SDS PAGE protein electrophoresis and Western blotting. |
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