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Anti-Rabbit anti GST-Tag Polyclonal Antibody (CABE067)

SKU:
CABE067
Product Type:
Antibody
Host Species:
Rabbit
Isotype:
IgG
Antibody Type:
Polyclonal Antibody
€169
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Description

system_update_altDatasheetsystem_update_altMSDS
Antibody Name:Anti-Rabbit anti GST-Tag Polyclonal Antibody
Antibody SKU:CABE067
Antibody Size:100 uL
Application:WB
Reactivity:
Host Species:Rabbit
Immunogen:Recombinant protein containing a sequence corresponding to amino acids 1-218 of GST protein.
Application:WB
Recommended Dilution:WB 1:500 - 1:1000
Reactivity:
Positive Samples:
Immunogen:Recombinant protein containing a sequence corresponding to amino acids 1-218 of GST protein.
Purification Method:Affinity purification
Storage Buffer:Store at 4?. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, pH7.3.
Isotype:IgG
Sequence:MSPI LGYW KIKG LVQP TRLL LEYL EEKY EEHL YERD EGDK WRNK KFEL GLEF PNLP YYID GDVK LTQS MAII RYIA DKHN MLGG CPKE RAEI SMLE GAVL DIRY GVSR IAYS KDFE TLKV DFLS KLPE MLKM FEDR LCHK TYLN GDHV THPD FMLY DALD VVLY MDPM CLDA FPKL VCFK KRIE AIPQ IDKY LKSS KYIA WPLQ GWQA TFGG GDHP PK
Gene ID:
Uniprot:
Cellular Location:
Calculated MW:26kDa
Observed MW:Refer to Figures
Anti-Rabbit anti GST-Tag Polyclonal Antibody (CABE067)Western blot - Rabbit anti GST-Tag pAb (CABE067)
Synonyms:GST, GST tag, GST-tag
Background:Glutathione S-transferases (GSTs), previously known as ligandins, comprise a family of eukaryotic and prokaryotic phase II metabolic isozymes best known for their ability to catalyze the conjugation of the reduced form of glutathione (GSH) to xenobiotic substrates for the purpose of detoxification. The GST family consists of three superfamilies: the cytosolic, mitochondrial, and microsomal€”also known as MAPEG€”proteins. Members of the GST superfamily are extremely diverse in amino acid sequence, and a large fraction of the sequences deposited in public databases are of unknown function. The Enzyme Function Initiative (EFI) is using GSTs as a model superfamily to identify new GST functions.A GST-tag is often used to separate and purify proteins that contain the GST-fusion protein. The tag is 220 amino acids (roughly 26 KDa) in size, which, compared to tags such as the Myc-tag or the FLAG-tag, is quite large. It can be fused to either the N-terminus or C-terminus of a protein. However, many commercially available sources of GST-tagged plasmids include a thrombin domain for cleavage of the GST tag during protein purification.

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