Anti-REC8 Antibody (CAB8660)

Antibody Name:	Anti-REC8 Antibody
Antibody SKU:	CAB8660
Antibody Size:	20uL, 50uL, 100uL
Application:	WB
Reactivity:	Human
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 1-270 of human REC8 (NP_005123.2).

Application:	WB
Recommended Dilution:	WB 1:500 - 1:2000
Reactivity:	Human
Positive Samples:	SGC-7901

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 1-270 of human REC8 (NP_005123.2).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	MFYY PNVL QRHT GCFA TIWL AATR GSRL VKRE YLRV NVVK TCEE ILNY VLVR VQPP QPGL PRPR FSLY LSAQ LQIG VIRV YSQQ CQYL VEDI QHIL ERLH RAQL QIRI DMET ELPS LLLP NHLA MMET LEDA PDPF FGMM SVDP RLPS PFDI PQIR HLLE AAIP ERVE EIPP EVPT EPRE PERI PVTV LPPE AITI LEAE PIRM LEIE GERE LPEV SRRE LDLL IAEE EEAI LLEI PRLP PPAP AEVE GIGE ALGP EELR LTGW EPGA LL
Gene ID:	9985
Uniprot:	O95072
Cellular Location:	Chromosome, Nucleus
Calculated MW:	60kDa/62kDa
Observed MW:	63kDa

Synonyms:

REC8, HR21spB, REC8L1, Rec8p

Background:

This gene encodes a member of the kleisin family of SMC (structural maintenance of chromosome) protein partners. The protein localizes to the axial elements of chromosomes during meiosis in both oocytes and spermatocytes. In the mouse, the homologous protein is a key component of the meiotic cohesion complex, which regulates sister chromatid cohesion and recombination between homologous chromosomes. Multiple alternatively spliced variants, encoding the same protein, have been found for this gene.

NCBI Summary:	This gene encodes a member of the kleisin family of SMC (structural maintenance of chromosome) protein partners. The protein localizes to the axial elements of chromosomes during meiosis in both oocytes and spermatocytes. In the mouse, the homologous protein is a key component of the meiotic cohesion complex, which regulates sister chromatid cohesion and recombination between homologous chromosomes. Multiple alternatively spliced variants, encoding the same protein, have been found for this gene. [provided by RefSeq, Jul 2008]
UniProt Code:	O95072
NCBI GenInfo Identifier:	14790109
NCBI Gene ID:	9985
NCBI Accession:	BC010887
UniProt Secondary Accession:	O95072,Q658V5, Q6IA92, Q8WUV8, Q9BTF2, Q9NVQ9, A8K576 D3DS62,
UniProt Related Accession:	O95072
Molecular Weight:	60,774 Da
NCBI Full Name:	Homo sapiens REC8 homolog (yeast), mRNA
NCBI Synonym Full Names:	REC8 meiotic recombination protein
NCBI Official Symbol:	REC8
NCBI Official Synonym Symbols:	Rec8p; REC8L1; HR21spB
NCBI Protein Information:	meiotic recombination protein REC8 homolog
UniProt Protein Name:
Protein Family:	Meiotic recombination protein
UniProt Gene Name:

Western blot analysis of extracts of SGC-7901 cells, using REC8 antibody (CAB8660) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 30s.

Antibodies

REC8 Antibody (PACO05862)

REC8 Antibody (PACO11790)

Secondary Antibody

Anti-HRP Goat Anti-Rabbit IgG (H+L) Antibody (CABS014)