Anti-SMPX Antibody (CAB6744)

Antibody Name:	Anti-SMPX Antibody
Antibody SKU:	CAB6744
Antibody Size:	20uL, 50uL, 100uL
Application:	WB IF
Reactivity:	Human, Mouse, Rat
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 1-88 of human SMPX (NP_055147.1).

Application:	WB IF
Recommended Dilution:	WB 1:500 - 1:2000 IF 1:50 - 1:100
Reactivity:	Human, Mouse, Rat
Positive Samples:	Mouse heart, Rat heart

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 1-88 of human SMPX (NP_055147.1).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	MNMS KQPV SNVR AIQA NINI PMGA FRPG AGQP PRRK ECTP EVEE GVPP TSDE EKKP IPGA KKLP GPAV NLSE IQNI KSEL KYVP KAEQ
Gene ID:	23676
Uniprot:	Q9UHP9
Cellular Location:
Calculated MW:	9kDa
Observed MW:	13kDa

Synonyms:	SMPX, DFN6, DFNX4
Background:	This gene encodes a small protein that has no known functional domains. Mutations in this gene are a cause of X-linked deafness-4, and the encoded protein may play a role in the maintenance of inner ear cells subjected to mechanical stress. Alternatively spliced transcript variants have been observed for this gene.

UniProt Protein Function:	SMPX: Plays a role in the regulatory network through which muscle cells coordinate their structural and functional states during growth, adaptation, and repair. Defects in SMPX are the cause of deafness X-linked type 4 (DFNX4). A non-syndromic form of sensorineural, progressive hearing loss with postlingual onset. In affected males, the auditory impairment affects initially high-frequency hearing. It later evolves to become severe to profound and affects all frequencies. Carrier females manifest moderate hearing impairment in the high frequencies. Belongs to the SMPX family.
UniProt Protein Details:	Protein type:Motility/polarity/chemotaxis Chromosomal Location of Human Ortholog: Xp22.1 Cellular Component: muscle tendon junction; costamere; M band; nucleus Biological Process: striated muscle contraction Disease: Deafness, X-linked 4
NCBI Summary:	This gene encodes a small protein that has no known functional domains. Mutations in this gene are a cause of X-linked deafness-4, and the encoded protein may play a role in the maintenance of inner ear cells subjected to mechanical stress. Alternatively spliced transcript variants have been observed for this gene. [provided by RefSeq, Dec 2011]
UniProt Code:	Q9UHP9
NCBI GenInfo Identifier:	239938852
NCBI Gene ID:	23676
NCBI Accession:	Q9UHP9.3
UniProt Secondary Accession:	Q9UHP9,B1AWX2,
UniProt Related Accession:	Q9UHP9
Molecular Weight:	88
NCBI Full Name:	Small muscular protein
NCBI Synonym Full Names:	small muscle protein, X-linked
NCBI Official Symbol:	SMPX
NCBI Official Synonym Symbols:	DFN6; DFNX4
NCBI Protein Information:	small muscular protein; deafness, X-linked 6, sensorineural; stretch-responsive skeletal muscle protein
UniProt Protein Name:	Small muscular protein
UniProt Synonym Protein Names:	Stretch-responsive skeletal muscle protein
Protein Family:	Small muscular protein
UniProt Gene Name:	SMPX
UniProt Entry Name:	SMPX_HUMAN

	Western blot analysis of extracts of various cell lines, using SMPX antibody (CAB6744) at 1:1000 dilution._Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution._Lysates/proteins: 25ug per lane._Blocking buffer: 3% nonfat dry milk in TBST._Detection: ECL Enhanced Kit (RM00021)._Exposure time: 90s.
	Immunofluorescence analysis of MCF7 cells using SMPX antibody (CAB6744). Blue: DAPI for nuclear staining.

Antibodies

Anti-SMPX Antibody (CAB13408)

SMPX Antibody (PACO12347)

Secondary Antibody

Anti-HRP Goat Anti-Rabbit IgG (H+L) Antibody (CABS014)