Anti-SPAG16 Antibody (CAB7482)

Antibody Name:	Anti-SPAG16 Antibody
Antibody SKU:	CAB7482
Antibody Size:	20uL, 50uL, 100uL
Application:	WB IHC
Reactivity:	Human, Mouse, Rat
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 1-183 of human SPAG16 (NP_001020607.1).

Application:	WB IHC
Recommended Dilution:	WB 1:500 - 1:2000 IHC 1:50 - 1:200
Reactivity:	Human, Mouse, Rat
Positive Samples:	Mouse testis

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 1-183 of human SPAG16 (NP_001020607.1).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	MAAQ RGMP SSAV RVLE EALG MGLT AAGD ARDT ADAV AAEG AYYL EQVT ITEA SEDD YEYE EIPD DNFS IPEG EEDL AKAI QMAQ EQAT DTEI LERK TVLP SKHA VPEV IEDF LCNF LIKM GMTR TLDC FQSE WYEL IQKG VTEL RTVG NVPD VYTQ IMLL ENEN KNLK KDLK HYKQ AAEY VIF
Gene ID:	79582
Uniprot:	Q8N0X2
Cellular Location:	Cytoplasm, cilium axoneme, cytoskeleton, flagellum axoneme
Calculated MW:	20kDa/29kDa/39kDa/50kDa/70kDa
Observed MW:	71kDa

Synonyms:	SPAG16, PF20, WDR29
Background:

UniProt Protein Function:	SPAG16: Necessary for sperm flagellar function. Plays a role in motile ciliogenesis. May help to recruit STK36 to the cilium or apical surface of the cell to initiate subsequent steps of construction of the central pair apparatus of motile cilia. 5 isoforms of the human protein are produced by alternative splicing.
UniProt Protein Details:	Chromosomal Location of Human Ortholog: 2q34 Cellular Component: axoneme Biological Process: axoneme biogenesis; cilium biogenesis
NCBI Summary:	Cilia and flagella are comprised of a microtubular backbone, the axoneme, which is organized by the basal body and surrounded by plasma membrane. SPAG16 encodes 2 major proteins that associate with the axoneme of sperm tail and the nucleus of postmeiotic germ cells, respectively (Zhang et al., 2007 [PubMed 17699735]).[supplied by OMIM, Jul 2008]
UniProt Code:	Q8N0X2
NCBI GenInfo Identifier:	146329993
NCBI Gene ID:	79582
NCBI Accession:	Q8N0X2.2
UniProt Secondary Accession:	Q8N0X2,Q498B7, Q658W1, Q68DB3, Q6I9Z6, Q8N9C7, Q9H601
UniProt Related Accession:	Q8N0X2
Molecular Weight:	39,739 Da
NCBI Full Name:	Sperm-associated antigen 16 protein
NCBI Synonym Full Names:	sperm associated antigen 16
NCBI Official Symbol:	SPAG16
NCBI Official Synonym Symbols:	PF20; WDR29
NCBI Protein Information:	sperm-associated antigen 16 protein
UniProt Protein Name:	Sperm-associated antigen 16 protein
UniProt Synonym Protein Names:	Pf20 protein homolog
Protein Family:	Sperm-associated antigen 16 protein
UniProt Gene Name:	SPAG16
UniProt Entry Name:	SPG16_HUMAN

	Western blot analysis of extracts of mouse testis, using SPAG16 antibody (CAB7482) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 90s.
	Immunohistochemistry of paraffin-embedded human brain cancer using SPAG16 antibody (CAB7482) at dilution of 1:100 (40x lens).
	Immunohistochemistry of paraffin-embedded mouse brain using SPAG16 antibody (CAB7482) at dilution of 1:100 (40x lens).

Antibodies

SPAG16 Antibody (PACO12411)

Secondary Antibody

Anti-HRP Goat Anti-Rabbit IgG (H+L) Antibody (CABS014)