Anti-SPTB Antibody (CAB13048)

Antibody Name:	Anti-SPTB Antibody
Antibody SKU:	CAB13048
Antibody Size:	20uL, 50uL, 100uL
Application:	WB IHC
Reactivity:	Human, Mouse, Rat
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 2080-2180 of human SPTB (NP_001020029.1).

Application:	WB IHC
Recommended Dilution:	WB 1:500 - 1:2000 IHC 1:50 - 1:200
Reactivity:	Human, Mouse, Rat
Positive Samples:	K-562, Mouse brain

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 2080-2180 of human SPTB (NP_001020029.1).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	QIAE RPAE ETGP QEEE GETA GEAP VSHH AATE RTSP GEEE GTWP QNLQ QPPP PGQH KDGQ KSTG DERP TTEP LFKV LDTP LSEG DEPA TLPA PRDH GQSV Q
Gene ID:	6710
Uniprot:	P11277
Cellular Location:	Cytoplasm, cell cortex, cytoskeleton
Calculated MW:	242kDa/246kDa/267kDa
Observed MW:	256kDa

Synonyms:

SPTB, EL3, HS2, HSPTB1, SPH2, spectrin beta

Background:

This locus encodes a member of the spectrin gene family. Spectrin proteins, along with ankyrin, play a role in cell membrane organization and stability. The protein encoded by this locus functions in stability of erythrocyte membranes, and mutations in this gene have been associated with spherocytosis type 2, hereditary elliptocytosis, and neonatal hemolytic anemia. Alternatively spliced transcript variants have been described.

UniProt Protein Function:	SPTB1: Spectrin is the major constituent of the cytoskeletal network underlying the erythrocyte plasma membrane. It associates with band 4.1 and actin to form the cytoskeletal superstructure of the erythrocyte plasma membrane. Defects in SPTB are the cause of elliptocytosis type 3 (EL3). EL3 is a Rhesus-unlinked form of hereditary elliptocytosis, a genetically heterogeneous, autosomal dominant hematologic disorder. It is characterized by variable hemolytic anemia and elliptical or oval red cell shape. Defects in SPTB are the cause of spherocytosis type 2 (SPH2); also known as hereditary spherocytosis type 2 (HS2). Spherocytosis is a hematologic disorder leading to chronic hemolytic anemia and characterized by numerous abnormally shaped erythrocytes which are generally spheroidal. SPH2 is characterized by severe hemolytic anemia. Inheritance is autosomal dominant. Belongs to the spectrin family. 3 isoforms of the human protein are produced by alternative splicing.
UniProt Protein Details:	Protein type:Cytoskeletal; Motility/polarity/chemotaxis Chromosomal Location of Human Ortholog: 14q23-q24.2 Cellular Component: spectrin; cell surface; protein complex; cytoplasm; cytosol; actin cytoskeleton Molecular Function:actin filament binding; protein binding; structural constituent of cytoskeleton; protein heterodimerization activity; ankyrin binding; actin binding Biological Process: axon guidance; plasma membrane organization and biogenesis; actin filament capping; hemopoiesis; porphyrin biosynthetic process Disease: Spherocytosis, Type 2, Included
NCBI Summary:	This locus encodes a member of the spectrin gene family. Spectrin proteins, along with ankyrin, play a role in cell membrane organization and stability. The protein encoded by this locus functions in stability of erythrocyte membranes, and mutations in this gene have been associated with spherocytosis type 2, hereditary elliptocytosis, and neonatal hemolytic anemia. Alternatively spliced transcript variants have been described. [provided by RefSeq, Nov 2009]
UniProt Code:	P11277
NCBI GenInfo Identifier:	67782319
NCBI Gene ID:	6710
NCBI Accession:	NP_000338.3
UniProt Secondary Accession:	P11277,Q15510, Q15519,
UniProt Related Accession:	P11277
Molecular Weight:	246,468 Da
NCBI Full Name:	spectrin beta chain, erythrocytic isoform b
NCBI Synonym Full Names:	spectrin, beta, erythrocytic
NCBI Official Symbol:	SPTB
NCBI Official Synonym Symbols:	EL3; HS2; SPH2; HSPTB1
NCBI Protein Information:	spectrin beta chain, erythrocytic; beta-I spectrin; membrane cytoskeletal protein; spectrin beta chain, erythrocyte
UniProt Protein Name:	Spectrin beta chain, erythrocytic
UniProt Synonym Protein Names:	Beta-I spectrin
Protein Family:	Spectrin
UniProt Gene Name:	SPTB
UniProt Entry Name:	SPTB1_HUMAN

Western blot analysis of extracts of various cell lines, using SPTB antibody (CAB13048) at 1:3000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit (RM00021). Exposure time: 90s.

Secondary Antibody

Anti-HRP Goat Anti-Rabbit IgG (H+L) Antibody (CABS014)