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Anti-USE1 Antibody (CAB13151)

SKU:
CAB13151
Product Type:
Antibody
Reactivity:
Human
Mouse
Host Species:
Rabbit
Isotype:
IgG
Antibody Type:
Polyclonal Antibody
Research Area:
Cell Biology
  • Cell Biology Antibodies 3 Anti-USE1 Antibody CAB13151
  • Cell Biology Antibodies 3 Anti-USE1 Antibody CAB13151
€139 - €419
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Description

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Antibody Name:Anti-USE1 Antibody
Antibody SKU:CAB13151
Antibody Size:20uL, 50uL, 100uL
Application:WB IF
Reactivity:Human, Mouse
Host Species:Rabbit
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 1-259 of human USE1 (NP_060937.1).
Application:WB IF
Recommended Dilution:WB 1:1000 - 1:2000 IF 1:50 - 1:200
Reactivity:Human, Mouse
Positive Samples:A-549, U-87MG, MCF7
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 1-259 of human USE1 (NP_060937.1).
Purification Method:Affinity purification
Storage Buffer:Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:IgG
Sequence:MAAS RLEL NLVR LLSR CEAM AAEK RDPD EWRL EKYV GALE DMLQ ALKV HASK PASE VINE YSWK VDFL KGML QAEK LTSS SEKA LANQ FLAP GRVP TTAR ERVP ATKT VHLQ SRAR YTSE MRSE LLGT DSAE PEMD VRKR TGVA GSQP VSEK QSAA ELDL VLQR HQNL QEKL AEEM LGLA RSLK TNTL AAQS VIKK DNQT LSHS LKMA DQNL EKLK TESE RLEQ HTQK SVNW LLWA MLII VCFI FISM ILFI RIMP KLK
Gene ID:55850
Uniprot:Q9NZ43
Cellular Location:Endoplasmic reticulum membrane, Single-pass type IV membrane protein
Calculated MW:15kDa/16kDa/29kDa
Observed MW:29kDa
Synonyms:USE1, D12, MDS032, P31, SLT1
Background:
UniProt Protein Function:USE1L: SNARE that may be involved in targeting and fusion of Golgi-derived retrograde transport vesicles with the ER. Belongs to the USE1 family. 3 isoforms of the human protein are produced by alternative splicing.
UniProt Protein Details:

Protein type:Vesicle; Membrane protein, integral

Chromosomal Location of Human Ortholog: 19p13.11

Cellular Component: endoplasmic reticulum; endoplasmic reticulum membrane; lysosome

Molecular Function:protein binding

Biological Process: ER to Golgi vesicle-mediated transport; lysosomal transport; protein catabolic process; retrograde vesicle-mediated transport, Golgi to ER; secretion by cell

UniProt Code:Q9NZ43
NCBI GenInfo Identifier:296452858
NCBI Gene ID:55850
NCBI Accession:Q9NZ43.2
UniProt Secondary Accession:Q9NZ43,Q8NCK1, Q9BRT4,
UniProt Related Accession:Q9NZ43
Molecular Weight:15,063 Da
NCBI Full Name:Vesicle transport protein USE1
NCBI Synonym Full Names:unconventional SNARE in the ER 1
NCBI Official Symbol:USE1  
NCBI Official Synonym Symbols:D12; P31; SLT1; MDS032  
NCBI Protein Information:vesicle transport protein USE1
UniProt Protein Name:Vesicle transport protein USE1
UniProt Synonym Protein Names:Putative MAPK-activating protein PM26; USE1-like protein; p31
Protein Family:Protein transport protein
UniProt Gene Name:USE1  
UniProt Entry Name:USE1_HUMAN
Anti-USE1 Antibody (CAB13151)Western blot analysis of extracts of various cell lines, using USE1 antibody (CAB13151) at 1:3000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 30s.
Anti-USE1 Antibody (CAB13151)Immunofluorescence analysis of NIH-3T3 cells using USE1 Polyclonal Antibody (CAB13151) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

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