Fibrosis Biomarker Assays

Fibrosis

Fibrosis, or fibrotic scarring, is a form of misguided pathological wound healing in which connective tissue replaces parenchymal tissue following repetitive injury or chronic inflammation. It involves a range of dysregulated signaling pathways across multiple cell types such as fibroblasts, myofibroblasts, epithelial cells and macrophages. If left unchecked, whole-scale tissue remodeling and scarring occurs. It follows accumulation of Extracellular Matric (ECM) components, such as collagen. Ultimately, it results in the thickening of the affected tissue, interfering with normal tissue function. It is commonly seen in tissues particularly susceptible to damage and inflammation such as the liver, lungs, heart and brain.

Assay Genie have developed a range of assays to detect fibrosis biomarkers, and changes in signaling associated with fibrosis. Below are a range of Human ELISA for popular biomarkers. Contact us for different species options or for activity assays and antibodies.

Fibrosis-Related Biomarkers

Non-specific Biomarkers of Fibrosis


Liver Fibrosis - ELISA Kits & Cellular Assays

ELISA Kits for Biomarkers of Liver Fibrosis

Biomarkers for specific detection of liver fibrosis include direct markers of extracellular matrix turnover and indirect markers that reflect liver dysfunction.

Cellular Assays for Liver Fibrosis

ELISA Kits for Biomarkers of Cardiac Fibrosis

ELISA Kits for Biomarkers of Lung Fibrosis

Animal Models of Fibrosis - ELISA Kits

Cell Signaling in Fibrosis

It is important to understand the molecular basis of the signaling pathways contributing to the activation and maintenance of myofibroblasts, the key cells in the pathophysiology of fibrotic disorders. At Assay Genie, we have launched Cell-based ELISA Kits & Transcription Factor Activity Assays for key the cell-signaling pathways mentioned below, related to the pathophysiology of fibrosis.
Several signal transduction pathways have been linked to the pathophysiology of fibrosis such as:

  • TGF-β
  • WNT
  • YAP/TAZ
  • MAPK/ERK
  • PI3K/AKT

Fibrosis Transcription Factor Activity Assays

At Assay Genie, we have launched Transcription Factor Activity Assays to detect the activation of key transcription factors in fibrosis.

Cell-Based ELISA Kits

Signaling Pathway Fibrosis Cell-Based ELISA Kits

At Assay Genie, we have launched Cell-Based ELISA Kits to screen for the effects of inhibitors & activators on protein expression in cell culture.

Multiplex Assays - Cytokine Release & Inflammation

At Assay Genie, we have launched our GeniePlex Multiplex Assay that can measure up to 24 cytokines & extracellular matrix proteins of fibrosis in every well! GeniePlex multiplex assays are a bead-based multiplex immunoassay technology that enables the simultaneous & quantitative detection of up to 24 analytes in a single 15ul sample on almost any flow cytometer.

GeniePlex Multiplex Assay - Key Features

  • Quantitative Immunoassays | Measure up to 24 analytes in a single 15ul sample
  • Validated Panels | Validated pre-made & custom panels
  • Comprehensive Choice | Choose from over 400 analytes from Human, Mouse, Rat, Non-Human Primate & Canine species
  • Inexpensive Operation | Runs on almost any flow cytometer
  • Optimized Samples | Tested on Serum, Plasma, Cell Culture Supernatant & tissues
  • Highly Sensitive | Sensitivity less than 10.0 pg/mL for most assays
  • Rapid Protocol | Assay Time approx. 4 hours
  • Precise Technology | Intra-Assay CV: <10%, Inter-Assay < 20%
  • Easy analysis | Easy analysis using commonly available software
  • Excellent Citation | Click here to view citation from University of Oxford

Antibodies

At Assay Genie, we have launched an extensive range of antibodies for a wide range of applications:

  • Western Blot (WB)
  • Flow Cytometry (FC)
  • Immunoprecipitation (IP)
  • Immunofluorescence (IF)
  • Immunohistochemistry (IHC)
  • Chromatin Immunoprecipitation (CHIP)